Recombinant Human IL-17C (CHO-expressed) Protein Summary
Product Specifications
His19-Val197
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9640-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
9640-IL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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When Recombinant Human IL-17 RE Chimera (Catalog # 8358-MR) is immobilize at 0.06 µg/mL, Recombinant Human IL-17C (Catalog # 9640-IL) binds with an ED50 of 0.2-1.2 ng/mL.
Reconstitution Calculator
Background: IL-17C
Interleukin-17C (IL-17C) is a 15-20 kDa glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17C shares 79% and 76% amino acid sequence identity with mouse and rat IL-17C, respectively (3). IL-17C binds to IL-17 RE with high affinity and to IL-17 RA with low affinity (4, 5). These two receptor chains can associate into a heterodimeric receptor for IL-17C (4-6). IL-17 RE is expressed on keratinocytes, mucosal epithelial cells, Th17 cells, and g/d T cells, while IL-17 RA is widely expressed (4, 5). IL-17 RE is required for mediating the pro-inflammatory and homeostatic actions of IL-17C in the skin and mucosa (1, 2). IL-17C expression is induced by inflammatory stimulation in colon and airway epithelial cells, keratinocytes, CD4+ T cells, macrophages, and dendritic cells (4, 6-9). It is up-regulated in various chronic inflammatory diseases including psoriasis, cystic fibrosis, and chronic obstructive pulmonary disease (COPD) (7, 8, 10). IL-17 RE is reciprocally down-regulated in psoriatic lesions (10). The interaction of IL-17C with IL-17 RE promotes mucosal immunity through the induction of anti-bacterial peptides and pro‑inflammatory cytokines and chemokines (4, 6, 8, 9). IL-17C action supports the integrity of the colon epithelium following infection induced damage (4, 6, 11) but also contributes to psoriatic skin thickening and the progression of arthritis (4, 8, 9). IL-17C is additionally upregulated in Th17 cell dependent autoimmunity (5). In this setting, it exacerbates disease severity by inducing Th17 cell production of IL-17A, IL-17F, IL-22, CCR6, and CCL20 (5).
- Pappu, R. et al. (2012) Trends Immunol. 33:343.
- Rubino, S.J. et al. (2012) Trends Immunol. 33:112.
- Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
- Ramirez-Carrozzi, V. et al. (2011) Nat. Immunol. 12:1159.
- Chang, S.H. et al. (2011) Immunity 35:611.
- Song, X. et al. (2011) Nat. Immunol. 12:1151.
- Pfeifer, P. et al. (2012) Am. J. Respir. Cell Mol. Biol. 48:415.
- Johnston, A. et al. (2013) J. Immunol. 190:2252.
- Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
- Johansen, C. et al. (2009) Br. J. Dermatol. 160:319.
- Reynolds, J.M. et al. (2012) J. Immunol. 189:4226.
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