What is the difference between the Recombinant Human IL-17F Proteins, Catalog #s 1335-IL and 1335-INS?

Name: Recombinant Human IL-17F Protein
Brand: R&D Systems
SKU: 1335-IL
Rating: 4.7 (3 reviews)

The main difference between these proteins is that 1335-IL is an E.coli-derived protein, while 1335-INS is an NS0-derived protein. The activity of both proteins is measured in a bioassay which induces IL-6 secretion by NIH 3T3 mouse embryonic fibroblast cells. We do observe a difference in ED50 activity for this effect, and the most likely explanation for this is the absence of post-translational modifications (PTMs) in 1335-IL.

Recombinant Human IL-17F Protein

Catalog #: 1335-IL
9 Citations 3 Reviews Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
1335-IL-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
1335-IL-025

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Product Details

Citations (9)

FAQs

Reviews (3)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17F Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 8-40 ng/mL.

Source

E. coli-derived human IL-17F protein
Arg31-Gln163, with an N-terminal Met

Accession #

Q96PD4

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

15 kDa (monomer)

Product Datasheets

1335-IL (with carrier)

Product Datasheet

COA

SDS

1335-IL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1335-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1335-IL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCI.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-17F

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Human IL-17F cDNA encodes a 163 aa protein with a putative 30 aa signal peptide. Among IL-17 family members, IL-17F is most closely related to IL-17A (approximately 44% aa sequence homology), but shares only limited sequence homology (16 - 30%) with IL-17B, C, D and E. Human and mouse IL-17F share 55% sequence identity. IL-17F is expressed in activated CD4⁺ T-cells and activated monocytes. Five receptors (IL-17 RA, B, C, D and E) have been identified (5). Although the ligands for IL-17 RD and E are not known yet, it is reported that IL-17 RA binds IL-17A, and IL-17 RB binds IL-17B and IL-17E. IL-17 RC binds IL‑17A and IL‑17F with similarly high affinity and functions as a receptor for both IL-17A and IL-17F (5, 6). The biological activities mediated by IL-17F are similar to those of IL-17. IL-17F stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (4). IL-17F also inhibits angiogenesis and induces production of IL-2, TGF-beta, and monocyte chemoattractant protein-1 in endothelial cells (3).

References

Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
Starnes, T. et al. (2001) J. Immunol. 167:4137.
Shen, F. & S. L. Gaffen (2008) Cytokine 41:92.
Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.

Long Name

Interleukin 17F

Entrez Gene IDs

112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)

Alternate Names

Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; interleukin 17F

Related Research Areas

Citations for Recombinant Human IL-17F Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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IL-17 cytokines preferentially act on naï¿½ve CD4+ T cells with the IL-17AF heterodimer inducing the greatest functional changes
Authors: MP Crawford, N Borcherdin, NJ Karandikar
PLoS ONE, 2023-04-28;18(4):e0285166.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
IL-17 cytokines preferentially act on naï¿½ve CD4+ T cells with the IL-17AF heterodimer inducing the greatest functional changes
Authors: MP Crawford, N Borcherdin, NJ Karandikar
PLoS ONE, 2023;18(4):e0285166.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
IL-17F induces IL-6 via TAK1-NF?B pathway in airway smooth muscle cells
Authors: M Nakajima, M Kawaguchi, K Ota, J Fujita, S Matsukura, SK Huang, Y Morishima, Y Ishii, H Satoh, T Sakamoto, N Hizawa
Immun Inflamm Dis, 2017-03-03;5(2):124-131.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Cytokines TNF-?, IL-6, IL-17F, and IL-4 Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells.
Authors: Angela P Bastidas-C, Astrid D Bakker, Behrouz Zandieh-D, Cornelis J Kleverlaa, Nathalie Bravenboe, Tim Forouzanf, Jenneke Klein-Nul
Stem Cells International, 2016-09-07;0(0):1687-966X.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
Authors: Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A
J Exp Med, 2015-04-27;212(5):619-31.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines.
Authors: Kisand K, Boe Wolff AS, Podkrajsek KT, Tserel L, Link M, Kisand KV, Ersvaer E, Perheentupa J, Erichsen MM, Bratanic N, Meloni A, Cetani F, Perniola R, Ergun-Longmire B, Maclaren N, Krohn KJ, Pura M, Schalke B, Strobel P, Leite MI, Battelino T, Husebye ES, Peterson P, Willcox N, Meager A
J. Exp. Med., 2010-02-01;207(2):299-308.
Species: Human
Sample Types: Serum
Applications: ELISA (Capture)
Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells.
Authors: Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB
Allergy, 2009-11-25;65(5):581-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
J. Immunol., 2009-03-01;182(0):3112-20.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis.
Authors: Watanabe H, Kawaguchi M, Fujishima S, Ogura M, Matsukura S, Takeuchi H, Ohba M, Sueki H, Kokubu F, Hizawa N, Adachi M, Huang SK, Iijima M
J. Invest. Dermatol., 2008-10-02;129(3):650-6.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

FAQs

What is the difference between the Recombinant Human IL-17F Proteins, Catalog #s 1335-IL and 1335-INS?
- The main difference between these proteins is that 1335-IL is an E.coli-derived protein, while 1335-INS is an NS0-derived protein. The activity of both proteins is measured in a bioassay which induces IL-6 secretion by NIH 3T3 mouse embryonic fibroblast cells. We do observe a difference in ED50 activity for this effect, and the most likely explanation for this is the absence of post-translational modifications (PTMs) in 1335-IL.

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