Recombinant Human IL-18 R alpha/IL-1 R5 Fc Avi-tag, CF

Catalog #: AVI10841 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI10841-050

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All IL-18 R alpha/IL-1 R5 Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-18 R alpha/IL-1 R5 Fc Avi-tag, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-18 R beta/IL-1 R7 Fc (Catalog # 118-AP) is immobilized at 2.00 µg/mL (100 µL/well), in the present of Recombinant Human IL-18 (Catalog # 9124-IL), Biotinylated Recombinant Human IL-18 R alpha/IL-1R5 Fc Chimera Avi-tag (Catalog # AVI10841) binds with an ED₅₀ of 0.500-5.00 ng/mL. The biotin to protein ratio is great than 0.7 as determined by the HABA assay.

Source

Human embryonic kidney cell, HEK293-derived human IL-18 R alpha/IL-1 R5 protein

Human IL-18 Ralpha/IL-1 R5 (Glu20-Arg329) Accession # Q13478.1	IEGRDID	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

Q13478.1

N-terminal Sequence
Analysis

Glu20

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Predicted Molecular Mass

64 kDa

SDS-PAGE

81-97 kDa, under reducing conditions

Product Datasheets

AVI10841

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10841

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera (118-AP) is immobilized at 2.00 μg/mL (100 μL/well), in the presnce of Recombinant Human IL-18 (9124-IL), Biotinylated Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera Avi-tag (Catalog # AVI10841) binds with an ED₅₀ of 0.500-5.00 ng/mL

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera Avi-tag Protein (Catalog # AVI10841) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 81-97 kDa and 162-194 kDa, respectively.

Reconstitution Calculator

Background: IL-18 R alpha/IL-1 R5

IL-18 Receptor alpha (IL-18 Ra), also known as IL-18 R1, IL-1 R5, and IL-1 Rrp, is a transmembrane component of the IL-18 Receptor (1). It associates with IL‑18 Rb/IL‑1 R7 to form the high affinity receptor complex for IL-18 and confer cellular responsiveness to IL-18 (2, 3). IL-18 is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. Mature human IL-18 Ra consists of a 308 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 191 aa cytoplasmic domain (4). Within the ECD, human IL-18 Ra shares 56% aa sequence identity with mouse and rat IL-18 Ra. IL-18 Ra is widely expressed on epithelial cells as well as on B cells, NK cells, CD4+ and CD8+ T cells, and dendritic cells (5-8). Signaling through the IL-18 receptor promotes a Th1 biased immune response and NK cell cytolytic activity (6, 7, 9-11). It also contributes to cigarette smoke-induced airway inflammation and epithelial damage (12). Our Avi-tag Biotinylated human IL-18 Ra features biotinylation at a single site contained within the avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound the streptavidin-coated surface due to the precise control of biotinylatin and the rest of the protein in changed so there is no interference in the protein's bioactivity.

References

Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
Kunikata, T. et al. (1998) Cell Immunol. 189:135.
Smeltz, R.B. et al. (2001) J. Exp. Med. 194:143.
Chan, W.L. et al. (2001) J. Immunol. 167:1238.
Kaser, A. et al. (2004) Blood 103:648.
Tomura, M. et al. (1998) J. Immunol. 160:3759.
Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
Hyodo, Y. et al. (1999) J. Immunol. 162:1662.
Kang, M.J. et al. (2007) J. Immunol. 178:1948.

Long Name

Interleukin 18 Receptor alpha

Entrez Gene IDs

8809 (Human); 16182 (Mouse)

Alternate Names

CD218a; CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra