Recombinant Human IL-18 R beta/IL-1 R7 His Avi Protein, CF

Catalog #: AVI11210 Datasheet / COA / SDS

Biotinylated

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AVI11210-050

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Recombinant Human IL-18 R beta/IL-1 R7 His-tag Avi-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-18 R beta/IL-1 R7 His Avi Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera (Catalog # 816-LR) is coated at 0.500 µg/mL (100 µL/well), Biotinylated Recombinant Human IL‑18R beta /IL‑1R7 His-tag Avi-tag (Catalog # AVI11210) binds with an ED50 of 2.00-40.0 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human IL-18 R beta/IL-1 R7 protein

Human IL-18 R beta/IL-1 R7 (Phe20-Arg356) Accession # O95256.1	6-His tag	Avi-tag
N-terminus		C-terminus

Accession #

O95256.1

N-terminal Sequence
Analysis

Phe20

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

47 kDa

SDS-PAGE

48-61 kDa, under reducing conditions.

Product Datasheets

AVI11210

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11210

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. When Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera Protein (816-LR) is coated at 0.500 µg/mL (100 µL/well), Biotinylated Recombinant Human IL‑18R beta /IL‑1R7 His-tag Avi-tag Protein (Catalog # AVI11210) binds with an ED₅₀ of 2.00-40.0 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human IL-18 R beta/IL-1 R7 His-tag Avi-tag Protein (Catalog # AVI11210) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-61 kDa.

Reconstitution Calculator

Background:

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon. Our Avi-tag Biotinylated IL-18 R beta /IL-1R7His-tag features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References