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Recombinant Human IL-19 (NS0-expressed) Protein

Catalog #: 9286-IL Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
9286-IL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
9286-IL-025
Recombinant Human IL-19 (NS0-expressed) Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-19 (NS0-expressed) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is 0.1-0.6 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-19 protein
Leu25-Ala177
Accession #
AAN40906
N-terminal Sequence
Analysis
Leu25
Predicted Molecular Mass
18 kDa
SDS-PAGE
20-30 kDa, reducing conditions

Product Datasheets

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9286-IL (with carrier)

Product Datasheet
COA
SDS

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9286-IL/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9286-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9286-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human IL-19 (NS0-expressed) Protein Bioactivity View Larger

Recombinant Human IL-19 (Catalog # 9286-IL) induces proliferation of BaF3 mouse pro-B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is 0.1-0.6 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-19

Interleukin 19 (IL-19) is a member of the IL-10 family of class II alpha -helical cytokines that contains two groups, a viral homolog and cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R beta as a signal transducing receptor (IL-10, IL-22, and IL-26), and one which uses IL-20 R beta as a signal transducing receptor (IL-19, IL-20, and IL-24) (1). The 153 amino acid (aa) mature human IL-19 is secreted as a glycosylated 35-45 kDa monomer (2-4). It shares approximately 70% aa sequence identity with mouse and rat IL-19. Human IL-19 may utilize an alternate start site that adds 38 aa to the N-terminus. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (2). IL-19 is expressed on activated keratinocytes, monocytes, and B cells (3, 5, 6). It binds a receptor complex consisting of IL-20 R alpha and IL-20 R beta (3, 7, 8). This receptor complex is also shared by IL-20 and IL-24 (9, 10). It has been reported that IL-19 both will and will not induce IL-6 and TNF-alpha production by monocytes (2, 11). It does, however, drive T helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (2, 11, 12).

References
  1. Commins, S. et al. (2008) J. Allergy Clin. Immunol. 121:1108.
  2. Liao, Y-C. et al. (2002) J. Immunol. 169:4288.
  3. Gallagher, G. et al. (2000) Genes Immun. 1:442.
  4. Pletnev, S. et al. (2003) Biochemistry 42:12617.
  5. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
  6. Wolk, K. et al. (2002) J. Immunol. 168:5397.
  7. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  8. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517.
  9. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
  10. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
  11. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
  12. Liao, S-C. et al. (2004) J. Immunol. 173:6712.
Long Name
Interleukin 19
Entrez Gene IDs
29949 (Human); 329244 (Mouse)
Alternate Names
IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19

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