Recombinant Human IL-2 R beta Fc Chimera Avi-tag Protein, CF

Catalog #: AVI10919 Datasheet / COA / SDS

Biotinylated

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AVI10919-025

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-2 R beta Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human IL-2 R beta Fc Chimera Avi-tag (Catalog # AVI10919) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human IL-15 (Catalog # 247-ILB) binds with an ED₅₀ of 0.50-4.00 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human IL-2 R beta protein

Human IL-2 R beta (Ala27-Asp239) Accession # P14784.1	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

P14784.1

N-terminal Sequence
Analysis

Ala27

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

53 kDa

SDS-PAGE

60-70 kDa, under reducing conditions.

Product Datasheets

AVI10919

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10919

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Biotinylated Recombinant Human IL-2 R beta Fc Chimera Avi-tag (Catalog # AVI10919) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human IL-15 (247-ILB) binds with an ED₅₀ of 0.50-4.00 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human IL-2 R beta Fc Chimera Avi-tag Protein (Catalog # AVI10919) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-70 kDa and 120-140 kDa, respectively.

Reconstitution Calculator

Background: IL-2 R beta

Interleukin-2 receptor subunit beta (IL-2 RB), also known as high affinity IL-2 receptor subunit beta, interleukin-15 receptor subunit beta, p70-75 (p75), and CD122, is a member of the cytokine receptor superfamily that plays a role in T cell-mediated immune responses (1, 3). Human IL-2 RB consists of an extracellular domain (ECD) containing a fibronectin type III domain and a WSXWS motif, a type I transmembrane domain, and a cytoplasmic tail. Within the ECD, mature human IL-2 RB shares 58% and 61% amino acid sequence identity with mouse and rat IL-2 RB, respectively. A soluble IL-2 RB (sIL-2 RB) has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 RB (5). Functional IL-2 receptors can exist in two affinity states on cell surfaces: the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains and the intermediate affinity complex comprising heterodimers of the beta and gamma chains (2, 3). Individual beta chains and alpha chains exhibit low affinity IL-2 binding and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling (4). At present, the function of IL-2 sR beta is unclear. Recombinant human sIL-2RB binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, sIL-2 RB binds IL-15 with sufficient affinity to neutralize IL-15 biological activities. Our Avi-tag Biotinylated Recombinant Human IL‑2 RB features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Jia, Z. et al. (2019) Int. Immunpharmacol. 74:105716.
Zhou, X. et al. (2019) Fish & Shellfish Immunol. 93:641.
Zhang, Z. et al. (2019) J. Exp. Med. 216:1311.
Fernandez, I. et al. (2019) J. Exp. Med. 216:1255.
Honda, M. et al. (1990) J. Immunol. 145:4131.

Long Name

Interleukin 2 Receptor beta

Entrez Gene IDs

3560 (Human); 16185 (Mouse)

Alternate Names

CD122 antigen; CD122; high affinity IL-2 receptor beta subunit; High affinity IL-2 receptor subunit beta; IL-15 R beta; IL-2 R beta; IL-2 receptor subunit beta; IL2R beta; IL-2R subunit beta; IL2RB; IL-2Rb; interleukin 15 receptor, beta; interleukin 2 receptor, beta; interleukin-2 receptor subunit beta; P70-75; p75