Recombinant Human IL-21R His-tag Protein, CF Summary
Product Specifications
Cys20-Pro236, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9249-R2
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Measured by its ability to inhibit IL-21-dependent enhancement of IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 15-150 ng/mL.
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Background: IL-21R
Interleukin-21 Receptor (IL-21 R )is a type I transmembrane glycoprotein within the class I cytokine receptor family (1). IL-21 R associates with the common gamma chain ( gamma c) which is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15 (2, 3). Mature human IL-21 R consists of a 213 amino acid (aa) extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSxWS motif, followed by a 21 aa transmembrane domain and a 285 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). Within the ECD, human IL-21 R shares 69% aa identity with mouse and rat IL-21 R, respectively. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 4, 5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (6). B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation) and is important for memory responses (7, 8). IL-21 R engagement enhances NK cell mediated cytotoxic activity and IFN-gamma production (4, 9), control of viral infection and tumor growth by CD8+ T cells (10), development of regulatory T cells (11), IL-23 responsiveness of encephalitogenic Th17 cells (12), but suppresses the accumulation of IL-17 secreting gamma δ T cells in the airway (13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (14, 15).
- Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107.
- Asao, H. et al. (2001) J. Immunol. 167:1.
- Habib, T. et al. (2002) Biochemistry 41:8725.
- Parrish-Novak, et al. (2000) Nature 408:57.
- Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
- Ozaki, K. et al. (2002) Science 298:1630.
- Rankin, A.L. et al. (2011) J. Immunol. 186:667.
- King, I.L. et al. (2010) J. Immunol. 185:6138.
- Kasaian, M.T. et al. (2002) Immunity 16:559.
- Frohlich, A. et al. (2009 Science 324:1576.
- Tortola, L. et al. (2010) Blood 116:5200.
- Lee, Y. et al. (2015) J. Clin. Invest. 125:4011.
- Moser, E.K. et al. (2015) PLoS One 10:e0120169.
- Jin, H. et al. (2009) J. Clin. Invest. 119:47.
- Sarosiek, K.A. et al. (2010) Blood 115:570.
Citation for Recombinant Human IL-21R His-tag Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Recruitment of plasma cells from IL-21-dependent and IL-21-independent immune reactions to the bone marrow
Authors: Ferreira-Gomes, M;Chen, Y;Durek, P;Rincon-Arevalo, H;Heinrich, F;Bauer, L;Szelinski, F;Guerra, GM;Stefanski, AL;Niedobitek, A;Wiedemann, A;Bondareva, M;Ritter, J;Lehmann, K;Hardt, S;Hipfl, C;Hein, S;Hildt, E;Matz, M;Mei, HE;Cheng, Q;Dang, VD;Witkowski, M;Lino, AC;Kruglov, A;Melchers, F;Perka, C;Schrezenmeier, EV;Hutloff, A;Radbruch, A;Dörner, T;Mashreghi, MF;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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