Recombinant Human IL-22 Avi (HEK293-expressed) Protein, CF

Catalog #: AVI11311 Datasheet / COA / SDS

Avi-tag

Catalog #	Availability	Size / Price	Qty
AVI11311-050

2 Images

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-22 Avi (HEK293-expressed) Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human IL-22 Avi-tag (Catalog # AVI11311) binds Recombinant Human IL-22BP (Catalog # 8498-BP/CF) with an ED₅₀ of 10.0‑120 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human IL-22 protein

GG	Avi-tag	Human IL-22 (Ala34-Ile179) Accession # Q9GZX6.1
N-terminus		C-terminus

Accession #

Q9GZX6.1

N-terminal Sequence
Analysis

Gly of GG Avi-tag

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

19 kDa

SDS-PAGE

25-31 kDa, under reducing conditions.

Product Datasheets

AVI11311

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11311

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

View Larger

Recombinant Human IL‑22 Avi-tag Protein (Catalog # AVI11311) binds Recombinant Human IL-22BP (8498-BP/CF) with an ED₅₀ of 10.0‑120 ng/mL.

SDS-PAGE

View Larger

2 μg/lane of Recombinant Human IL‑22 Avi-tag Protein (Catalog # AVI11311) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 25-31 kDa, under reducing conditions.

Reconstitution Calculator

Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT‑1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family. Our Avi-tag Biotinylated human IL-22 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
Dumoutier, L. et al. (2000) PNAS 97:10144.
Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Long Name

Interleukin 22

Entrez Gene IDs

50616 (Human); 50929 (Mouse); 500836 (Rat)

Alternate Names

Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18