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Recombinant Human IL-28A/IFN-lambda 2 (HEK293) Protein

Catalog #: 8417-IL
4 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
8417-IL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
8417-IL-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (4)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-28A/IFN-lambda 2 (HEK293) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED50 for this effect is 5-25 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human IL-28A/IFN-lambda 2 protein
Val26-Val200
Accession #
Q8IZJ0
N-terminal Sequence
Analysis
Val26 & Val28
Predicted Molecular Mass
20 kDa
SDS-PAGE
20-23 kDa, reducing condtions

Product Datasheets

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8417-IL (with carrier)

Product Datasheet
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SDS

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8417-IL/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8417-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8417-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-28A/IFN-lambda 2

IL‑28A (Interferon-lambda 2; IFN-lambda 2), IL-28B/IFN-lambda 3, and IL-29/IFN-lambda 1 are type III interferons which are distantly related to IL-10 family and type I IFN family cytokines (1‑3). Mature human IL-28A is an approximately 22-25 kDa protein that shares 66% amino acid (aa) sequence identity with mouse and rat IL-28A and shows cross-species activity (4). It shares 96% and 70% aa sequence identity with human IL-28B and IL-29, respectively. Type III interferons are upregulated in response to viral infection, and they act predominantly on epithelial cells through a receptor complex that contains IL-10 R beta and IL-28 R alpha /IFN-gamma R1 (4-6). Type III IFNs function as anti-viral molecules and induce the upregulation of MHC class I antigen (4, 5, 7). IL-28A promotes the Th1 polarization of dendritic cells in the airway (e.g. production of IL-12 p70 and IFN-gamma ) and inhibits Th2 and Th17 mediated inflammation (8). In the liver, however, the IL-28A induced Th1 cytokine response contributes to inflammation in T cell mediated hepatitis (9). IL-28A additionally exhibits anti-tumor activity, in part by enhancing IL-12 dependent anti-tumor CTL responses in vivo (10-12). In contrast, it is up-regulated in invasive bladder cancer where it promotes tumor cell migration (13).

References
  1. Durbin, R.K. et al. (2013) Immunol. Rev. 255:25.
  2. Li, Q. et al. (2013) Front. Biosci. (Landmark Ed.) 18:909.
  3. Lopusna, K. et al. (2013) Acta Virol. 57:171.
  4. Ank, N. et al. (2006) J. Virol. 80:4501.
  5. Sheppard, P. et al. (2003) Nature Immunol. 4:63.
  6. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.
  7. Pott, J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:7944.
  8. Koltsida, O. et al. (2011) EMBO Mol. Med. 3:348.
  9. Siebler, J. et al. (2007) Gastroenterology 132:358.
  10. Sato, A. et al. (2006) J. Immunol. 176:7686.
  11. Tezuka, Y. et al. (2012) Lung Cancer 78:185.
  12. Numasaki, M. et al. (2007) J. Immunol. 178:5086.
  13. Lee, S.J. et al. (2012) PLoS ONE 7:e40267.
Long Name
Interleukin 28A
Entrez Gene IDs
282616 (Human)
Alternate Names
interleukin-28A; IFN-lambda 2; IL28A; IL-28A; interferon, lambda 2

Citations for Recombinant Human IL-28A/IFN-lambda 2 (HEK293) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Highly sensitive reporter cell line for detection of interferon types I-III and their neutralization by antibodies
    Authors: Groen, K;Kuratli, R;Sar, L;Vasou, A;Huber, M;Hughes, DJ;Hale, BG;
    European journal of immunology
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: Bioassay
  2. LRRC15 inhibits SARS-CoV-2 cellular entry in trans
    Authors: J Song, RD Chow, M Pena-Herna, L Zhang, SA Loeb, EY So, OD Liang, P Ren, S Chen, CB Wilen, S Lee
    PloS Biology, 2022-10-13;20(10):e3001805.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Structure-based glycoengineering of interferon lambda 4 enhances its productivity and anti-viral potency
    Authors: JH Chung, SH Hong, N Seo, TS Kim, HJ An, P Lee, EC Shin, HM Kim
    Cytokine, 2019-08-31;125(0):154833.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. IFN-?4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling
    Authors: AA Obajemu, N Rao, KA Dilley, JM Vargas, F Sheikh, RP Donnelly, RS Shabman, EG Meissner, L Prokunina-, OO Onabajo
    J. Immunol., 2017-10-25;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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