Recombinant Human IL-28A/IFN-lambda 2 Protein Summary
Product Specifications
| Human IL-28A Val26-Val200 Accession # NP_742150 |
DI | 6-His tag |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1587-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1587-IL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-28A/IFN-lambda 2
IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11-13% aa sequence identity) and type I IFN family (15-19% aa sequence identity) (1-3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon. The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory regions of the target genes. Human IL-28A cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 25 aa signal peptide. It shares 94% and 67% aa sequence identity with human IL-28B and human IL-29, respectively.
Citations for Recombinant Human IL-28A/IFN-lambda 2 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Interferon-&lambda Enhances the Differentiation of Naive B Cells into Plasmablasts via the mTORC1 Pathway
Authors: M Syedbasha, F Bonfiglio, J Linnik, C Stuehler, D Wüthrich, A Egli
Cell Rep, 2020-10-06;33(1):108211.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interferon lambda receptor 1 (IFNL1R) transcript is highly expressed in rhinovirus bronchiolitis and correlates with disease severity
Authors: A Pierangeli, M Statzu, R Nenna, L Santinelli, L Petrarca, A Frassanito, M Gentile, G Antonelli, F Midulla, C Scagnolari
J. Clin. Virol., 2018-03-10;102(0):101-109.
Species: Human
Sample Types: Whole Cells
Applications: Stimulation -
Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface
Authors: J Corry, N Arora, CA Good, Y Sadovsky, CB Coyne
Proc. Natl. Acad. Sci. U.S.A., 2017-08-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IFN-?4 potently blocks IFN-? signalling by ISG15 and USP18 in hepatitis C virus infection
Authors: PS Sung, SH Hong, JH Chung, S Kim, SH Park, HM Kim, SK Yoon, EC Shin
Sci Rep, 2017-06-19;7(1):3821.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Hepatitis-C-virus-induced microRNAs dampen interferon-mediated antiviral signaling
Nat. Med., 2016-11-14;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Respiratory syncytial virus can infect basal cells and alter human airway epithelial differentiation.
Authors: Persson B, Jaffe A, Fearns R, Danahay H
PLoS ONE, 2014-07-17;9(7):e102368.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Epigenetic reprogramming of the type III interferon response potentiates antiviral activity and suppresses tumor growth.
Authors: Ding S, Khoury-Hanold W, Iwasaki A, Robek M
PLoS Biol, 2014-01-07;12(1):e1001758.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-28A triggers wound healing migration of bladder cancer cells via NF-kappaB-mediated MMP-9 expression inducing the MAPK pathway.
Cell. Signal., 2012-04-25;24(9):1734-42.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Adenoviruses-mediated transduction of human oesophageal carcinoma cells with the interferon-lambda genes produced anti-tumour effects.
Authors: Li Q, Kawamura K, Okamoto S, Fujie H, Numasaki M, Namba M, Nagata M, Shimada H, Kobayashi H, Tagawa M
Br. J. Cancer, 2011-09-27;105(9):1302-12.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
Authors: Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K
Genes Immun., 2009-10-01;10(8):702-14.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
Int. J. Cancer, 2009-07-15;125(2):367-73.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Biological activity of interleukins-28 and -29: comparison with type I interferons.
Authors: Meager A, Visvalingam K, Dilger P, Bryan D, Wadhwa M
Cytokine, 2005-07-21;31(2):109-18.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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This recombinant IFN-lambda 2 protein was combined with IFN-lambda 1 (1598-IL) to serve as the calibrator in a pan specific IFN-lambda assay using MAB15981 and BAF1598 as the matched pair antibodies.
