Home / IL-36 alpha/IL-1F6 / Recombinant Human IL-36 alpha/IL-1F6 (aa 6-158) Protein

Recombinant Human IL-36 alpha/IL-1F6 (aa 6-158) Protein

Catalog #: 6995-IL
5 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
6995-IL-010/CF

With Carrier

Catalog # Availability Size / Price Qty
6995-IL-010
Recombinant Human IL-36 alpha/IL-1F6 (aa 6-158) Protein Bioactivity
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Product Details
Citations (5)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-36 alpha/IL-1F6 (aa 6-158) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 4-24 ng/mL.
Source
E. coli-derived human IL-36 alpha/IL-1F6 protein
Lys6-Phe158
Accession #
NP_055255
N-terminal Sequence
Analysis
Lys6
Structure / Form
Monomer
Predicted Molecular Mass
17 kDa
SDS-PAGE
16 kDa, reducing conditions

Product Datasheets

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6995-IL (with carrier)

Product Datasheet
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6995-IL/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6995-IL

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA and Tween® 20 with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in Water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6995-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA and Tween® 20 with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in Water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human IL-36 alpha/IL-1F6 (aa 6-158) Protein Bioactivity View Larger

Recombinant Human IL-36 alpha/IL-1F6 (Catalog # 6995-IL) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 4-24 ng/mL.

SDS-PAGE Recombinant Human IL-36 alpha/IL-1F6 (aa 6-158) Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human IL-36 alpha /IL-1F6 (aa 6-158) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 16 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-36 alpha/IL-1F6

Human IL-36 alpha, previously called IL-1F6 and FIL1 epsilon  (family of IL-1 member epsilon), is a member of the IL-1 family which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18, and novel family members IL-36 Ra (IL-1F5), IL-36 beta (IL-1F8), IL-36 gamma (IL-1F9), IL-37 (IL-1F7) and IL‑38 (IL‑1F10) (1‑4). All family members show a 12  beta ‑strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene (1, 2). IL-36 alpha is an 18‑22 kDa, 158 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential from N‑linked glycosylation sites (1‑3). It can be released in response to LPS and the cell ATP‑induced activation of the P2X7 receptor (5). A 120 aa isoform missing aa 1‑38 has been reported (6). Human IL‑36 alpha (aa 6 ‑ 158) shares 57‑68% aa sequence identity with mouse, rabbit, equine and bovine IL‑36 alpha and 27‑57% aa sequence identity with other novel IL‑1 family members. IL‑36 alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine (1, 3, 7). It is expressed by monocytes, B and T cells (1, 2). The receptor for IL‑36 alpha is a combination of IL‑1 Rrp2 (also called IL1RL2 or IL‑1 R6), mainly found in epithelia and keratinocytes, and the widely expressed IL‑1 RAcP (3, 7). IL-36 alpha, beta, and gamma all activate NF-kappa B and MAPK pathways in an IL‑1 Rrp2 dependent manner, and induce production of inflammatory cytokines and chemokines such as CXCL8/IL-8 (7). IL-36 alpha and other family members are overexpressed in psoriatic skin lesions, and transgenic overexpression of IL‑36 alpha in skin keratinocytes produces epidermal hyperplasia (7‑9). IL-36 alpha is present in kidney tubule epithelia, and it is highly expressed in intubulointerstitial lesions in mouse models of chronic glomerulonephritis, lupus nephritis and diabetic nephritis (10).

References
  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  3. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  4. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
  5. Martin, U. et al. (2009) J. Immunol. 183:4021.
  6. Entrez Accession # EAW73614.
  7. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  8. Blumberg, H. et al. (2010) J. Immunol. 185:4354.
  9. Johnston, A. et al. (2011) J. Immunol. 186:2613.
  10. Ichii, O. et al. (2010) Lab. Invest. 90:459.
Long Name
Interleukin 36 alpha/Interleukin 1 Family 6
Entrez Gene IDs
27179 (Human); 54448 (Mouse)
Alternate Names
FIL1 epsilon; FIL1; FIL1(EPSILON); FIL1E; IL-1 epsilon; IL1(EPSILON); IL1E; IL-1E; IL-1F6 (FIL-1-epsilon); IL1F6; IL-1F6; IL36 alpha; IL-36 alpha; IL36A; interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin 36, Alpha; Interleukin-1 epsilon; interleukin-1 family member 6; Interleukin-36 Alpha

Citations for Recombinant Human IL-36 alpha/IL-1F6 (aa 6-158) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. The CDK4/6-EZH2 pathway is a potential therapeutic target for psoriasis
    Authors: A Müller, A Dickmanns, C Resch, K Schäkel, S Hailfinger, M Dobbelstei, K Schulze-Os, D Kramer
    J. Clin. Invest., 2020-11-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. IL-36? regulates mediators of tissue homeostasis in epithelial cells
    Authors: JE Heath, GM Scholz, PD Veith, EC Reynolds
    Cytokine, 2019-03-08;119(0):24-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. RNA-Seq Analysis of IL-1B and IL-36 Responses in Epidermal Keratinocytes Identifies a Shared MyD88-Dependent Gene Signature
    Authors: WR Swindell, MA Beamer, MK Sarkar, S Loftus, J Fullmer, X Xing, NL Ward, LC Tsoi, MJ Kahlenberg, Y Liang, JE Gudjonsson
    Front Immunol, 2018-01-29;9(0):80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Structural and functional attributes of the Interleukin-36 receptor
    Authors: Guanghui Yi
    J Biol Chem, 2016-06-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Signal Transduction and Intracellular Trafficking by the Interleukin 36 Receptor.
    Authors: Saha S, Singh D, Raymond E, Ganesan R, Caviness G, Grimaldi C, Woska J, Mennerich D, Brown S, Mbow M, Kao C
    J Biol Chem, 2015-08-12;290(39):23997-4006.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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