Recombinant Human IL-36Ra/IL-1F5 Protein Summary
Product Specifications
Val2-Asp155
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1275-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1275-IL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant Human IL-36Ra/IL-1F5 (Catalog # 1275-IL) inhibits Recombinant Human IL-36 beta/IL-1F8 (Catalog # 6834-ILB) induced IL-8 secretion by A431 human epithelial carcinoma cells. The ED50 for this effect is 0.2-1 μg/mL.
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Background: IL-36Ra/IL-1F5
Human interleukin-36 receptor antagonist [IL-36Ra; previously IL-1F5 and also named FIL-1δ (delta), IL‑1HY1, IL‑1H3, and IL-1L1] is a member of the IL‑1 family of proteins (1 ‑ 6). IL‑1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL‑18 and IL-1F5-F10 (7). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that underwent multiple duplications (7). The human IL‑36Ra/IL‑1F5 gene is in closest proximity to the gene for IL-1ra and is likely a relatively recent duplication of the IL-1ra gene (2, 3). IL-36Ra/IL-1F5 is synthesized as a 155 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2 - 5). Nevertheless, it appears to be secreted as a 17 kDa monomer (5). There is an alternate start site that potentially gives rise to an alternate splice form (5). The translated product, however, has a premature stop codon, resulting in a truncated 16 aa peptide. Human to mouse, full length IL-1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 50% aa identical to IL-1ra, and 32%, 31%, 35%, 37%, 32% and 42% aa identical to IL-1 beta, IL-36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL‑36 beta /IL‑1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express
IL‑36Ra/IL‑1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 8). The receptor for
IL-36Ra/IL-1F5 has not been positively identified. Indirect evidence suggests it is IL-1 Rrp2 and/or IL-1 RAcP (9). In either case, activity association with receptor binding is also unclear. It was initially reported to be an antagonist of IL‑36 gamma /IL‑1F9 activity (4, 6). This would be consistent with its hypothesized relationship to
IL‑1ra. Studies, however, find IL-36Ra/IL-1F5 antagonist activity difficult to demonstrate (9).
- Smith, D. E. et al. (2000) J. Biol. Chem. 275:1169.
- Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
- Mulero, J.J. et al. (1999) Biochem. Biophys. Res. Commun. 263:702.
- Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
- Barton, J.L. et al. (2000) Eur. J. Immunol. 30:3299.
- Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
- Dunn, E. et al. (2001) Trends Immunol. 22:533.
- Debets, R. et al. (2001) J. Immunol. 167:1440.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Citations for Recombinant Human IL-36Ra/IL-1F5 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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IL-36G promotes cancer-cell intrinsic hallmarks in human gastric cancer cells
Authors: N Le, I Luk, D Chisanga, W Shi, L Pang, G Scholz, J Mariadason, M Ernst, J Huynh
Cytokine, 2022-05-02;155(0):155887.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin (IL)-17/IL-36 axis participates to the crosstalk between endothelial cells and keratinocytes during inflammatory skin responses
Authors: L Mercurio, CM Failla, L Capriotti, C Scarponi, F Facchiano, M Morelli, S Rossi, G Pagnanelli, C Albanesi, A Cavani, S Madonna
PLoS ONE, 2020-04-30;15(4):e0222969.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-38 has an anti-inflammatory action in psoriasis and its expression correlates with disease severity and therapeutic response to anti-IL-17A treatment
Authors: L Mercurio, M Morelli, C Scarponi, EZ Eisenmesse, N Doti, G Pagnanelli, E Gubinelli, C Mazzanti, A Cavani, M Ruvo, CA Dinarello, C Albanesi, S Madonna
Cell Death Dis, 2018-10-30;9(11):1104.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-36 receptor mediates the crosstalk between plasma cells and synovial fibroblasts
Authors: V Schmitt, M Hahn, V Kästele, O Wagner, M Wiendl, A Derer, A Taddeo, S Hahne, A Radbruch, HM Jäck, W Schuh, D Mielenz, S Gay, G Schett, AJ Hueber, S Frey
Eur. J. Immunol., 2017-09-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-36 induces cytokine IL-6 and chemokine CXCL8 expression in human lung tissue cells: Implications for pulmonary inflammatory responses
Authors: J Zhang, Y Yin, X Lin, X Yan, Y Xia, L Zhang, J Cao
Cytokine, 2017-09-03;99(0):114-123.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Structural and functional attributes of the Interleukin-36 receptor
Authors: Guanghui Yi
J Biol Chem, 2016-06-15;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Signal Transduction and Intracellular Trafficking by the Interleukin 36 Receptor.
Authors: Saha S, Singh D, Raymond E, Ganesan R, Caviness G, Grimaldi C, Woska J, Mennerich D, Brown S, Mbow M, Kao C
J Biol Chem, 2015-08-12;290(39):23997-4006.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist.
Authors: van de Veerdonk FL, Stoeckman AK, Wu G, Boeckermann AN, Azam T, Netea MG, Joosten LA, Van der Meer JW, Hao R, Kalabokis V, Dinarello CA
Proc. Natl. Acad. Sci. U.S.A., 2012-02-06;109(8):3001-5.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay
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