Home / IL-37b/IL-1F7b / Recombinant Human IL-37b/IL-1F7b Protein

Recombinant Human IL-37b/IL-1F7b Protein

Catalog #: 7585-IL
9 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
7585-IL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
7585-IL-025
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Citations (9)
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Recombinant Human IL-37b/IL-1F7b Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance IL-18 BP inhibition of IL-18-induced IFN-gamma release by KG‑1 human acute myelogenous leukemia cells. Bufler, P. et al. (2002) PNAS 99:13723. The ED50 for this effect is 2.5-12.5 μg/mL.
Source
E. coli-derived human IL-37b/IL-1F7b protein
Val46-Asp218
Accession #
Q9NZH6
N-terminal Sequence
Analysis
Val46
Predicted Molecular Mass
19.4 kDa
SDS-PAGE
25 kDa, reducing conditions

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7585-IL (with carrier)

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7585-IL/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7585-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7585-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-37b/IL-1F7b

Human Interleukin 1 family member 7 (IL-1F7), also named FIL-1 zeta, IL-37, IL-1H4, IL-1HL and IL-1RP1, is an anti-inflammatory member of the IL-1 cytokine family. IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. Five isoforms (IL-1F7a through e) are generated through alternative exon usage and have distinct expression profiles (1‑5). Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript is the basis for the product offered here. This product, also referred to as IL-1F7b, or IL-1F7 isoform 1, is normally generated from a 218 amino acid (aa) proprotein that consists of a 45 aa propeptide plus a 173 aa mature region that is cleaved by caspase-1 to generate the 32 kDa mature form (aa 46-218) (6). IL-1F7 is up-regulated by inflammatory stimuli in peripheral blood mononuclear cells (7). Experimental overexpression of IL-1F7b in vivo limits the inflammatory response and protects mice from colitis and LPS-induced shock (7, 8). Both unprocessed and mature IL-1F7b can form homodimers in solution (6, 7). Although IL-1F7b will bind to IL-18 R alpha with low affinity, this has no effect on IL‑18 receptor activity (4, 6, 9). Alternatively, IL-1F7b will also bind to IL-18 BP, generating a complex that interacts with IL-18R beta. This has the effect of attenuating IL‑18 activity via the IL-18 receptor (9). IL-1F7b can also function intracellularly. Following LPS-induced cleavage of its propeptide, IL-1F7b associates with Smad3 and translocates to the nucleus. This results in a reduction of pro-inflammatory cytokine secretion (10).

References
  1. Smith, D.E. et al. (2000) J. Biol Chem 275:1169.
  2. Kumar S. et al. (2000) J. Biol Chem 275:10308.
  3. Busfield S.J. et al. (2000) Genomics 66:213.
  4. Pan, G. et al. (2000) Cytokine 13:1.
  5. Boraschi, D. et al. (2011) Eur. Cytokine Netw. 22:127.
  6. Kumar S. et al. (2002) Cytokine 18:61.
  7. Nold, M.F. et al. (2010) Nat. Immunol. 11:1014.
  8. McNamee, E.N. et al. (2011) Proc. Natl. Acad. Sci. USA 108:16711.
  9. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
  10. Sharma, S. et al. (2008) J. Immunol. 180:5477.
Long Name
Interleukin 37 Isoform B
Entrez Gene IDs
27178 (Human)
Alternate Names
IL37b; IL-37b

Citations for Recombinant Human IL-37b/IL-1F7b Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Plant-produced recombinant cytokines IL-37b and IL-38 modulate inflammatory response from stimulated human PBMCs
    Authors: I Kolotilin
    Scientific Reports, 2022-11-14;12(1):19450.
    Species: Human
    Sample Types:
    Applications: Bioassay
  2. A Splice Switch in SIGIRR Causes a Defect of IL-37-Dependent Anti-Inflammatory Activity in Cystic Fibrosis Airway Epithelial Cells
    Authors: K Ueno-Shuto, S Kamei, M Hayashi, A Fukuyama, Y Uchida, N Tokutomi, MA Suico, H Kai, T Shuto
    International Journal of Molecular Sciences, 2022-07-13;23(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. IL-37b alleviates endothelial cell apoptosis and inflammation in Kawasaki disease through IL-1R8 pathway
    Authors: C Jia, Y Zhuge, S Zhang, C Ni, L Wang, R Wu, C Niu, Z Wen, X Rong, H Qiu, M Chu
    Cell Death & Disease, 2021-06-03;12(6):575.
    Species: Human, Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  4. IL-38 inhibits microglial inflammatory mediators and is decreased in amygdala of children with autism spectrum disorder
    Authors: I Tsilioni, H Pantazopou, P Conti, SE Leeman, TC Theoharide
    Proc. Natl. Acad. Sci. U.S.A., 2020-06-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. IL-37 is increased in brains of children with autism spectrum disorder and inhibits human microglia stimulated by neurotensin
    Authors: I Tsilioni, AB Patel, H Pantazopou, S Berretta, P Conti, SE Leeman, TC Theoharide
    Proc. Natl. Acad. Sci. U.S.A., 2019-10-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Interleukin-37 Enhances the Suppressive Activity of Naturally Occurring CD4(+)CD25(+) Regulatory T Cells
    Sci Rep, 2016-12-12;6(0):38955.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Treating experimental arthritis with the innate immune inhibitor interleukin-37 reduces joint and systemic inflammation.
    Authors: Cavalli G, Koenders M, Kalabokis V, Kim J, Tan A, Garlanda C, Mantovani A, Dagna L, Joosten L, Dinarello C
    Rheumatology (Oxford), 2016-08-26;55(12):2220-2229.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma
    Sci Rep, 2016-05-26;6(0):26757.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Transfer of the IL-37b gene elicits anti-tumor responses in mice bearing 4T1 breast cancer.
    Authors: Wang W, Zhao D, Zhou Y, Hu X, Sun Z, Yu G, Wu W, Chen S, Kuang J, Xu G, Han Z, Wang B, Yang J, Feng X
    Acta Pharmacol Sin, 2015-04-01;36(4):528-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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