Recombinant Human IL-4R alpha Protein, CF

Catalog #: 230-4RB
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
230-4RB-025

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-4R alpha Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 2‑10 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL‑4 (Catalog # 204-IL).

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived human IL-4R alpha protein
Met1-His232

Accession #

P24394

N-terminal Sequence
Analysis

Gly24

Predicted Molecular Mass

24 kDa

SDS-PAGE

26-39 kDa, reducing conditions

Product Datasheets

230-4RB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

230-4RB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-4R alpha

Interleukin 4 Receptor alpha (IL‑4 R alpha ), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. IL‑4 R alpha plays an important role in Th2‑biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis (1‑5). Mature human IL‑4 R alpha consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a cytokine binding region and one fibronectin type‑III domain, a 24 aa transmembrane segment, and a 569 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif (6, 7). Within the ECD, human IL‑4 R alpha shares 51% aa sequence identity with mouse and rat IL‑4 R alpha. Soluble forms of IL‑4 R alpha, generated by alternate splicing or proteolysis, retain ligand binding properties and inhibit IL‑4 bioactivity (8‑11). IL‑4 R alpha is a component of two distinct receptor complexes and shows species selectivity between human and mouse (6). It can associate with the common gamma chain ( gamma c) to form the IL‑4 responsive type I receptor in which gamma c increases the affinity for IL‑4 and enables signaling (12, 13). It can alternatively associate with IL‑13 R alpha 1 to form the type II receptor which is responsive to both IL‑4 and IL‑13 (14, 15). The use of shared receptor components contributes to the overlapping biological effects of IL‑4 and IL‑13 as well as other cytokines that utilize gamma c (i.e. IL‑2, IL‑7, IL‑9, IL‑15, and IL‑21) (16, 17).

References

Wills-Karp, M. and F.D. Finkelman (2008) Sci. Signal. 1:pe55.
Gordon, S. and F.O. Martinez (2010) Immunity 32:593.
Kuperman, D.A. and R.P. Schleimer (2008) Curr. Mol. Med. 8:384.
Li, Z. et al. (2009) Cell. Mol. Immunol. 6:415.
Lee, Y.W. et al. (2010) Biomol. Ther. 18:135.
Idzerda, R.L. et al. (1990) J. Exp. Med. 171:861.
Galizzi, J.P. et al. (1990) Int. Immunol. 2:669.
Kruse, S. et al. (1999) Int. Immunol. 11:1965.
Blum, H. et al. (1996) J. Immunol. 157:1846.
Jung, T. et al. (1999) Int. Arch. Allergy Immunol. 119:23.
Mosley, B. et al. (1989) Cell 59:335.
Kondo, M. et al. (1993) Science 262:1874.
Russell, S.M. et al. (1993) Science 262:1880.
Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. 93:497.
Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
Ramalingam, T.R. et al. (2008) Nat. Immunol. 9:25.
Overwijk, W.W. and K.S. Schluns (2009) Clin. Immunol. 132:153.

Long Name

Interleukin 4 Receptor alpha

Entrez Gene IDs

3566 (Human); 16190 (Mouse); 102133649 (Cynomolgus Monkey)

Alternate Names

CD124 antigen; CD124; IL-4 R alpha; IL-4 receptor subunit alpha; IL4R alpha; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha

Related Research Areas

Citation for Recombinant Human IL-4R alpha Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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