Recombinant Human IL-4R alpha Protein Summary
Product Specifications
Gly24-His232
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
230-4R
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
230-4R/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-4R alpha
Interleukin 4 Receptor alpha (IL-4 R alpha ), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. IL-4 R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis (1 - 5). Mature human IL-4 R alpha consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a cytokine binding region and one fibronectin type-III domain, a 24 aa transmembrane segment, and a 569 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif (6, 7). Within the ECD, human IL-4 Ra shares 51% aa sequence identity with mouse and rat IL-4 R alpha. Soluble forms of IL-4 R alpha, generated by alternate splicing or proteolysis, retain ligand binding properties and inhibit IL-4 bioactivity (8 - 11). IL-4 R alpha is a component of two distinct receptor complexes and shows species selectivity between human and mouse (6). It can associate with the common gamma chain ( gamma c) to form the IL-4 responsive type I receptor in which gamma c increases the affinity for IL-4 and enables signaling (12, 13). It can alternatively associate with IL-13 R alpha 1 to form the type II receptor which is responsive to both IL-4 and IL-13 (14, 15). The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL‑13 as well as other cytokines that utilize gamma c (i.e. IL-2, IL-7, IL-9, IL-15, and IL-21) (16, 17).
- Wills-Karp, M. and F.D. Finkelman (2008) Sci. Signal. 1:pe55.
- Gordon, S. and F.O. Martinez (2010) Immunity 32:593.
- Kuperman, D.A. and R.P. Schleimer (2008) Curr. Mol. Med. 8:384.
- Li, Z. et al. (2009) Cell. Mol. Immunol. 6:415.
- Lee, Y.W. et al. (2010) Biomol. Ther. 18:135.
- Idzerda, R.L. et al. (1990) J. Exp. Med. 171:861.
- Galizzi, J.P. et al. (1990) Int. Immunol. 2:669.
- Kruse, S. et al. (1999) Int. Immunol. 11:1965.
- Blum, H. et al. (1996) J. Immunol. 157:1846.
- Jung, T. et al. (1999) Int. Arch. Allergy Immunol. 119:23.
- Mosley, B. et al. (1989) Cell 59:335.
- Kondo, M. et al. (1993) Science 262:1874.
- Russell, S.M. et al. (1993) Science 262:1880.
- Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. 93:497.
- Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
- Ramalingam, T.R. et al. (2008) Nat. Immunol. 9:25.
- Overwijk, W.W. and K.S. Schluns (2009) Clin. Immunol. 132:153.
Citations for Recombinant Human IL-4R alpha Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Extracellular and intracellular mechanisms of mechanotransduction in three-dimensionally embedded rat chondrocytes.
Authors: Shioji, Suguru, Imai, Shinji, Ando, Kosei, Kumagai, Kousuke, Matsusue, Yoshitak
PLoS ONE, 2014-12-05;9(12):e114327.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Effect of an interleukin-4 variant on late phase asthmatic response to allergen challenge in asthmatic patients: results of two phase 2a studies.
Authors: Wenzel S, Wilbraham D, Fuller R, Getz EB, Longphre M
Lancet, 2007-10-20;370(9596):1422-31.
Species: Human
Sample Types: Serum
Applications: Binding Assay -
IL-13 receptor alpha 2: a regulator of IL-13 and IL-4 signal transduction in primary human fibroblasts.
Authors: Andrews AL, Nasir T, Bucchieri F, Holloway JW, Holgate ST, Davies DE
J. Allergy Clin. Immunol., 2006-09-08;118(4):858-65.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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