Recombinant Human INSRR Protein, CF

Catalog #: 9566-RR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9566-RR-050

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human INSRR Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Chinese Hamster Ovary cell line, CHO-derived human INSRR protein

Human INSRR beta
(Ala747-Leu923)
Accession # P14616

HHHHHH

Human INSRR alpha
(Leu24-Gly742)
Accession # P14616

Human INSRR beta (Ala747-Leu923) Accession # P14616	HHHHHH
N-terminus	C-terminus

Accession #

P14616

N-terminal Sequence
Analysis

Leu24 (INSRR alpha ) & Ala747 (INSRR beta )

Structure / Form

Disulfide-linked heterotetramer

Predicted Molecular Mass

100 kDa

SDS-PAGE

30-39 kDa, 93 - 116 kDa, 124 - 144 kDa, reducing conditions

Product Datasheets

9566-RR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9566-RR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: INSRR

Insulin Receptor-related Receptor (INSRR) is a type I transmembrane glycoprotein in the Insulin/IGF receptor family of receptor tyrosine kinases (1). The mature receptor contains an extracellular alpha subunit and an extracellular/transmembrane/cytoplasmic beta subunit. The amino acid 1-923 encodes the extracellular domain. Alternative splicing generates A and B isoforms which may homodimerize or heterodimerize with the IGF-I receptor (2). INSRR signaling regulates glucose uptake and metabolism but also contributes to cell growth, differentiation and apoptosis. INSRR is specifically and reversibly activated by alkaline media through a positive cooperativity mechanism. It also triggers receptor conformational changes and elicits intracellular signaling. INSRR is predominantly expressed in organs that come in contact with mildly alkaline media, and is postulated to be an alkali sensor to manage metabolic bicarbonate excess in kidney (3). Mutations in INSRR have been linked to severe insulin resistance (type A and Rabson-Mendenhall syndrome), type II diabetes mellitus, and leprechaunism (Donohue syndrome) (4, 5). Human INSRR shares 89% identity with mouse and rat INSRR.

References