Recombinant Human Integrin alpha 5 beta 1 Fc Protein, CF New

Catalog #: 11538-A5 Datasheet / COA / SDS

Fc Chimera

Catalog #	Availability	Size / Price	Qty
11538-A5-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Integrin alpha 5 beta 1 Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human Integrin alpha 5 beta 1 Fc Chimera (Catalog # 11538-A5) binds Human Fibronectin (Catalog # 1918-FN) with an ED₅₀ of 0.600‑9.00 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human Integrin alpha 5 beta 1 protein

Human ITGA5 (Phe42-Tyr995) Accession # P08648.2	IEGR	Human IgG1 (Pro99-Lys330) (with modifications)
Human ITGB1 (Gln21-Asp728) Accession # P05556.2	IEGR	Human IgG1 (Pro99-Lys330) (with modifications)
N-terminus		C-terminus

N-terminal Sequence
Analysis

Phe 42 (Integrin alpha 5) & Gln21 inferred from enzymatic pyroglutamate treatment revelaing Ser 22 (Integrin beta 1)

Structure / Form

Disulfide-linked heterodimer

Predicted Molecular Mass

131 kDa (Integrin alpha 4) & 105 kDa (Integrin beta 1)

SDS-PAGE

225-370 kDa, under non-reducing conditions

Product Datasheets

11538-A5

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11538-A5

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Recombinant Human Integrin alpha 5 beta 1 Fc Chimera Protein (Catalog # 11538-A5) binds Human Fibronectin (1918-FN) with an ED₅₀ of 0.600‑9.00 ng/mL.

Reconstitution Calculator

Background: Integrin alpha 5 beta 1

Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). The human Integrin alpha 5/CD49e cDNA encodes a 1049 amino acid (aa) precursor that includes a 41 aa signal sequence, a 954 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain. The ECD contains seven FG-GAP repeats and nine internal disulfide bonds (3). Within the ECD, human alpha 5 shares 90% and 46% aa sequence identity with mouse alpha 5 and human alpha 8, respectively, and less than 30% aa sequence identitity with other human alpha chains. The human Integrin beta 1/CD29 cDNA encodes a 798 aa precursor that includes a 20 aa signal sequence, a 708 aa ECD, a 23 aa transmembrane segment, and a 47 aa cytoplasmic domain. The ECD contains one vWF-A domain, four Cys-rich repeats, and 29 internal disulfide bonds (3). Five alternate splice forms of the cytoplasmic domain vary by 12 to 48 aa. Within the ECD, human beta 1 shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. It shares 35-45% aa sequence identity with other beta chains. alpha 5/ beta 1 binds fibronectin in both RGD-dependent and -independent manners (4, 5). alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGFR3 and Tie2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including HER2, uPAR, Galectin‑1, CTGF, and thrombin-cleaved Osteopontin (5, 10-13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14, 15).

References

Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
Takagi, J. et al. (2003) EMBO J. 22:4607.
Wei, Y. et al. (2005) J. Cell Biol. 168:501.
Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.
Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
Cascone, I. et al. (2005) J. Cell Biol. 170:993.
Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
Gao, R. and D.R. Brigstock (2005) Gut 129:1019
Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
Carstanjen, D. et al. (2005) Transfusion 45:1192.
Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.

Entrez Gene IDs

3678 (Human)

Alternate Names

CD49 antigen-like family member E; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha polypeptide; fibronectin receptor, alpha subunit; FNRA; Integrin alpha 5 beta 1; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5/SLC9B1 fusion; very late activation protein 5, alpha subunit; VLA-5; VLA5A