Recombinant Human KAL1 Protein, CF Summary
Product Specifications
Ala25-Tyr680, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9314-KA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. |
| Reconstitution | Reconstitute at 200 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
View Larger
When Recombinant Human FGF basic 146 aa (Catalog # 233-FB) is coated at 1.5 µg/mL, Recombinant Human KAL1 (Catalog # 9314-KA) binds with an ED50 of 8-48 ng/mL.
Reconstitution Calculator
Background: KAL1
Anosmin-1, also known as Kal-1, is a 100 kDa secreted glycoprotein that regulates neuronal adhesion and migration. Mutations in Anosmin-1 are associated with the development of X-linked Kallman syndrome which can be accompanied by hearing loss (1). Mature human Anosmin-1 contains one WAP domain and four Fibronectin type-III domains (2). It is expressed by cerebellar Purkinje cells and deep nuclear neurons (2, 3), piriform cortex and olfactory bulb mitral cells (2, 4), optic tectum and retinal ganglion axons (2, 5, 6), and oligodendrocyte progenitor cells (OPC) (4). Anosmin-1 binds to Integrin beta 1/CD29, FGF R1, L1CAM, and complexes of FGF R1 with FGF basic and heparan sulfate proteoglycans, and it enhances signaling through FGF R1 (6-9). It mediates OPC adhesion, inhibits FGF basic-induced OPC migration, and enhances oligodendrocyte-mediated axon myelination (5, 6, 10, 11). It also cooperates with FGF basic to promote olfactory axon elongation and neurite outgrowth (3, 4, 8, 9, 12, 13). In addition, Anosmin-1 is up-regulated in glioblastoma and gastric cancer where it enhances tumor cell proliferation and migration (7, 14).
- Korsensky, L. and D. Ron (2016) Semin. Cell Dev. Biol. 53:101.
- Soussi-Yanicostas, N. et al. (1996) J. Cell Sci. 109:1749.
- Gianola, S. et al. (2009) Neuroscience 158:570.
- Soussi-Yanicostas, N. et al. (2002) Cell 109:217.
- Bribian, A. et al. (2008) Dev. Neurobiol. 68:1503.
- Bribian, A. et al. (2006) Mol. Cell. Neurosci. 33:2.
- Choy, C.T. et al. (2013) Endocr. Relat. Cancer 21:85.
- Diaz-Balzac, C.A. et al. (2015) Cell Rep. 11:1377.
- Gonzalez-Martinez, D. et al. (2004) J. Neurosci. 24:10384.
- Clemente, D. et al. (2011) J. Neurosci. 31:14899.
- Murcia-Belmonte, V. et al. (2016) Brain Struct. Funct. 221:1365.
- Soussi-Yanicostas, N. et al. (1998) J. Cell Sci. 111:2953.
- Garcia-Gonzalez, D. et al. (2016) Brain Struct. Funct. 221:239.
- Kanda, M. et al. (2016) Int. J. Cancer 138:721.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human KAL1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human KAL1 Protein, CF and earn rewards!
Have you used Recombinant Human KAL1 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
