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Recombinant Human KIR2DL1/CD158a Fc Chimera Protein, CF

Catalog #: 1844-KR
5 Citations Datasheet / COA / SDS
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1844-KR-050
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Citations (5)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human KIR2DL1/CD158a Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind KG‑1A human acute myelogenous leukemia cells in a flow cytometry assay. When 250 ng of Recombinant Human KIR2DL1/CD158a Fc Chimera is added to 1 x 106 KG-1A cells, >55% of the cells will bind to the protein.
Source
Mouse myeloma cell line, NS0-derived human KIR2DL1/CD158a protein
Human KIR2DL1/CD158a
(His22-Arg242)
Accession # P43626		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
P43626
N-terminal Sequence
Analysis
His22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51 kDa (monomer)
SDS-PAGE
72-80 kDa, reducing conditions

Product Datasheets

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1844-KR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1844-KR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KIR2DL1/CD158a

KIR2DL1 (2DL1, formerly NKAT1, designated CD158a) is a 348 amino acid (aa) type I transmembrane glycoprotein that belongs to the human killer cell Ig-like receptor (KIR) family (1, 2). KIRs are expressed on human CD56dim NK cells and T cell subsets, and regulate effector functions in the innate immune system (1-3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails (1-3). Individuals will express varying subsets of inhibiting and activating KIRs with varying polymorphisms (1, 4). Like other inhibiting KIRs, KIR2DL1 has two ITIM domains within its long tail that block activating receptor clustering (2, 5). Within the ECD, KIR2DL1 shares high aa sequence identity (92%) with KIR2DL2. KIR2DL1 targets Lys80-containing HLA-C2 allotypes while KIR2DL2 rather recognizes Ans80 containing HLA-C1 (1, 6-8). Three KIRs proteins together (2DL1-3) recognize and inhibit NK cytotoxicity against cells expressing any HLA-C allotype, allowing self-recognition, but also conferring susceptibility to leukemia (1-3). In primary human NK cell clones, KIR2DL1 regulates the level of MHC I proteins expression required for NK cell inhibition (9).

References
  1. Purdy, A.K. and Campbell, K.S. (2009) Cancer Biol. Ther. 8:13.
  2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  3. Kulkarni, S. et al. (2008) Sem. Immunol. 20:343.
  4. Middleton, D. and F. Gonzelez (2009) Immunology 129:8.
  5. Abeyweera, T.P. et al. (2011) J. Cell Biol. 192:675.
  6. Moesta, A.K. et al. (2008) J. Immunol. 180:3969.
  7. Boyington, J.C. et al. (2000) Nature 405:537.
  8. Snyder, G.A. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3864.
  9. Almeida, C.R. et al. (2011) PLoS ONE. 6:e24927.
Long Name
Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 1
Entrez Gene IDs
3802 (Human)
Alternate Names
CD158 antigen-like family member A; CD158a antigen; CD158a; CD158Ankat1; cl-42; killer cell immunoglobulin-like receptor 2DL1; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1; killer Ig receptor; killer inhibitory receptor 2-2-1; KIR221; KIR2DL1; MHC class I NK cell receptor; Natural killer-associated transcript 1; NKAT; NKAT-1; NKAT1KIR-K64; p58 killer cell inhibitory receptor KIR-K64; p58 natural killer cell receptor clones CL-42/47.11; p58 NK cell inhibitory receptor NKR-K6,47.11; p58 NK receptor CL-42/47.11; p58.1 MHC class-I-specific NK receptor; p58.1

Citations for Recombinant Human KIR2DL1/CD158a Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. HIV-1 Nef-mediated downregulation of CD155 results in viral restriction by KIR2DL5+ NK cells
    Authors: P Fittje, A Hœlzemer, WF Garcia-Bel, S Vollmers, A Niehrs, K Hagemann, G Martrus, C Körner, F Kirchhoff, D Sauter, M Altfeld
    Oncogene, 2022-06-24;18(6):e1010572.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  2. Human NK cell receptor KIR2DS4 detects a conserved bacterial epitope presented by HLA-C
    Authors: MJW Sim, S Rajagopala, DM Altmann, RJ Boyton, PD Sun, EO Long
    Proc. Natl. Acad. Sci. U.S.A., 2019-05-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. HLA-C-restricted viral epitopes are associated with an escape mechanism from KIR2DL2+ NK cells in Lassa virus infection
    Authors: N Wauquier, C Petitdeman, N Tarantino, C Maucourant, M Coomber, V Lungay, J Bangura, P Debré, V Vieillard
    EBioMedicine, 2019-01-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Peptide-specific engagement of the activating NK cell receptor KIR2DS1
    Authors: A Chapel, WF Garcia-Bel, A Hölzemer, M Ziegler, S Lunemann, G Martrus, M Altfeld
    Sci Rep, 2017-05-25;7(1):2414.
    Applications: Bioassay
  5. ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
    Authors: Cifaldi L, Romania P, Falco M, Lorenzi S, Meazza R, Petrini S, Andreani M, Pende D, Locatelli F, Fruci D
    Cancer Res, 2015-01-15;75(5):824-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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