Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF
Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF Summary
Product Specifications
| Human KIR2DL2 (His22 - His245) Accession # P43627 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3015-KR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: KIR2DL2/CD158b1
KIR2DL2 (2DL2, formerly NKAT6, designated CD158b) is a 348 amino acid (aa) type I transmembrane glycoprotein that belongs to the human killer cell Ig‑like receptor (KIR) family (1, 2). KIRs are expressed on human CD56dim NK cells and T cell subsets, and regulate effector functions in the innate immune system (1 ‑ 3). KIRs are named for the number of Ig‑like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails (1 ‑ 3). Individuals will express varying subsets of inhibiting and activating KIRs with varying polymorphisms (1, 4). Like other inhibiting KIRs, KIR2DL2 has two ITIM domains within its long tail that block activating receptor clustering (2, 5). Within the ECD, KIR2DL2 shares very high aa sequence identity (98%) with KIR2DL3. The two segregate as alleles of the same gene, although KIR2DL2 shows higher avidity for HLA‑C1 ligands (1, 6). Extracellular aa identity is also high for KIR2DL1 (92%). The three together recognize and inhibit NK cytotoxicity against cells expressing any HLA‑C allotype, allowing self‑recognition, but also conferring susceptibility to leukemia (1 ‑ 3). KIR2DL2 recognizes Asn80‑containing HLA‑C1 and, more weakly, Lys80‑containing C2 allotypes (1, 6 ‑ 8). KIR2DL2 can impact both innate and adaptive immunity, contributing to either viral persistence or antiviral immunity, depending on the HLA class I molecules expressed by the individual (9).
- Purdy, A.K. and Campbell, K.S. (2009) Cancer Biol. Ther. 8:13.
- Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
- Kulkarni, S. et al. (2008) Sem. Immunol. 20:343.
- Middleton, D. and F. Gonzelez (2009) Immunology 129:8.
- Abeyweera, T.P. et al. (2011) J. Cell Biol. 192:675.
- Moesta, A.K. et al. (2008) J. Immunol. 180:3969.
- Boyington, J.C. et al. (2000) Nature 405:537.
- Snyder, G.A. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3864.
- Seich al Basatena, N-K. et al. (2011) PLoS Pathog. 7:e1002270.
Citations for Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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HLA-C-restricted viral epitopes are associated with an escape mechanism from KIR2DL2+ NK cells in Lassa virus infection
Authors: N Wauquier, C Petitdeman, N Tarantino, C Maucourant, M Coomber, V Lungay, J Bangura, P Debré, V Vieillard
EBioMedicine, 2019-01-30;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Peptide selectivity discriminates NK cells from KIR2DL2- and KIR2DL3-positive individuals.
Authors: Cassidy S, Mukherjee S, Myint T, Mbiribindi B, North H, Traherne J, Mulder A, Claas F, Purbhoo M, Das J, Khakoo S
Eur J Immunol, 2014-11-29;45(2):492-500.
Applications: Bioassay -
HLA-Cw*0102-Restricted HIV-1 p24 Epitope Variants Can Modulate the Binding of the Inhibitory KIR2DL2 Receptor and Primary NK Cell Function.
Authors: Fadda L, Korner C, Kumar S, van Teijlingen NH, Piechocka-Trocha A, Carrington M, Altfeld M
PLoS Pathog., 2012-07-12;8(7):e1002805.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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