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Recombinant Human LIGHT/TNFSF14 Protein

Catalog #: 664-LI
21 Citations 2 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
664-LI-025/CF

With Carrier

Catalog # Availability Size / Price Qty
664-LI-025
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Recombinant Human LIGHT/TNFSF14 Protein Bioactivity
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Product Details
Citations (21)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LIGHT/TNFSF14 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 0.3-3 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human LIGHT/TNFSF14 protein
Met 10-His tag GGGSGGGSGGGSIEGR Human Light
(Asp74-Val240)
Accession # O43557
N-terminus C-terminus
Accession #
O43557
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
20.9 kDa
SDS-PAGE
25 kDa, reducing conditions

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664-LI (with carrier)

Product Datasheet
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664-LI/CF (carrier free)

Product Datasheet
COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

664-LI

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

664-LI/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human LIGHT/TNFSF14 Protein Bioactivity View Larger

Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-4 ng/mL.

SDS-PAGE Recombinant Human LIGHT/TNFSF14 Protein SDS-PAGE View Larger

1 µg/lane of Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 25 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: LIGHT/TNFSF14

Human LIGHT is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma. The full roles of LIGHT remain to be elucidated.

References
  1. Mauri, D.N. et al. (1998) Immunity 8:21.
  2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
  4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.
Long Name
TNF Ligand Superfamily Member 14
Entrez Gene IDs
8740 (Human); 50930 (Mouse)
Alternate Names
CD258 antigen; CD258; delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator ligand; HVEM-L; HVEMLherpesvirus entry mediator-ligand; ligand for herpesvirus entry mediator; LIGHT; LIGHTherpesvirus entry mediator A; LTg; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT

Citations for Recombinant Human LIGHT/TNFSF14 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Tumoral and stromal hMENA isoforms impact tertiary lymphoid structure localization in lung cancer and predict immune checkpoint blockade response in patients with cancer
    Authors: Di Modugno, F;Di Carlo, A;Spada, S;Palermo, B;D'Ambrosio, L;D'Andrea, D;Morello, G;Belmonte, B;Sperduti, I;Balzano, V;Gallo, E;Melchionna, R;Panetta, M;Campo, G;De Nicola, F;Goeman, F;Antoniani, B;Carpano, S;Frigè, G;Warren, S;Gallina, F;Lambrechts, D;Xiong, J;Vincent, BG;Wheeler, N;Bortone, DS;Cappuzzo, F;Facciolo, F;Tripodo, C;Visca, P;Nisticò, P;
    EBioMedicine
    Species: Human
    Sample Types: Transfected Whole Cells, Whole Cells
    Applications: Bioassay
  2. LIGHT (TNFSF14) promotes the differentiation of human bone marrow-derived mesenchymal stem cells into functional hepatocyte-like cells
    Authors: Heo, SK;Yu, HM;Kim, DK;Seo, HJ;Shin, Y;Kim, SA;Kim, M;Kim, Y;Lee, YJ;Noh, EK;Jo, JC;
    PloS one
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. PI16+ reticular cells in human palatine tonsils govern T cell activity in distinct subepithelial niches
    Authors: De Martin, A;Stanossek, Y;L�tge, M;Cadosch, N;Onder, L;Cheng, HW;Brandstadter, JD;Maillard, I;Stoeckli, SJ;Pikor, NB;Ludewig, B;
    Nature immunology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. LIGHT (TNFSF14) enhances osteogenesis of human bone marrow-derived mesenchymal stem cells
    Authors: SK Heo, Y Choi, YK Jeong, LJ Ju, HM Yu, DK Kim, HJ Seo, YJ Lee, J Cheon, S Koh, YJ Min, EK Noh, JC Jo
    PLoS ONE, 2021-02-19;16(2):e0247368.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. The Tumor Suppressor, p53, Negatively Regulates Non-Canonical NF-kappaB Signaling through miRNAInduced Silencing of NF-kappaB-Inducing Kinase.
    Authors: Jang H, Park S, Kim J, Kim J, Kim S, Cho S, Park S, Park B, Kim S, Kim J
    Mol Cells, 2020-01-31;43(1):23-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies
    Authors: S An, I Raju, B Surenkhuu, JE Kwon, S Gulati, M Karaman, A Pradeep, S Sinha, C Mun, S Jain
    Ocul Surf, 2019-04-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. TNFSF14 inhibits melanogenesis via NF-kB signaling in melanocytes
    Authors: KH Kim, H Choi, HJ Kim, TR Lee
    Cytokine, 2018-05-03;110(0):126-130.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. TNFSF14 (LIGHT) Exhibits Inflammatory Activities in Lung Fibroblasts Complementary to IL-13 and TGF-β
    Authors: R da Silva A, AK Mehta, L Madge, J Tocker, M Croft
    Front Immunol, 2018-03-19;9(0):576.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  9. An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
    Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
    MAbs, 2018-03-05;0(0):1-43.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  10. Interleukin-22 reverses human islet dysfunction and apoptosis triggered by hyperglycemia and LIGHT
    Authors: S Abadpour, B Halvorsen, A Sahraoui, O Korsgren, P Aukrust, H Scholz
    J. Mol. Endocrinol., 2018-01-12;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. LIGHT is increased in patients with coronary disease and regulates inflammatory response and lipid metabolism in oxLDL-induced THP-1 macrophages
    Authors: X Yuan, Y Gu, X Lai, Q Gu
    Biochem. Biophys. Res. Commun., 2017-06-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. A TNFRSF14-Fc?RI-mast cell pathway contributes to development of multiple features of asthma pathology in mice
    Nat Commun, 2016-12-16;7(0):13696.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. LIGHT (TNFSF14) Increases the Survival and Proliferation of Human Bone Marrow-Derived Mesenchymal Stem Cells
    PLoS ONE, 2016-11-11;11(11):e0166589.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
    Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
    J Immunol, 2015-07-24;195(5):2429-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis.
    Authors: Herro R, Antunes R, Aguilera A, Tamada K, Croft M
    J Invest Dermatol, 2015-03-19;135(8):2109-18.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Lymphotoxin beta receptor signaling induces IL-8 production in human bronchial epithelial cells.
    Authors: Mikami Y, Matsuzaki H, Horie M, Noguchi S, Jo T, Narumoto O, Kohyama T, Takizawa H, Nagase T, Yamauchi Y
    PLoS ONE, 2014-12-11;9(12):e114791.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-kappaB pathway.
    Authors: Jin HR, Jin X, Lee JJ
    Biochem. Biophys. Res. Commun., 2010-09-06;400(4):581-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Inflammatory interaction between LIGHT and proteinase-activated receptor-2 in endothelial cells: potential role in atherogenesis.
    Authors: Sandberg WJ, Halvorsen B, Yndestad A, Smith C, Otterdal K, Brosstad FR, Froland SS, Olofsson PS, Damas JK, Gullestad L, Hansson GK, Oie E, Aukrust P
    Circ. Res., 2008-11-20;104(1):60-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes.
    Authors: Kang YM, Kim SY, Kang JH
    Arthritis Rheum., 2007-04-01;56(4):1106-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
    Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
    Blood, 2006-08-01;108(3):928-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis.
    Authors: Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A
    Arthritis Rheum., 2006-05-01;54(5):1451-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human LIGHT/TNFSF14 Protein

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Recombinant Human LIGHT/TNFSF14 Protein
By Anonymous on 03/10/2021
Application: Stem/Immune cell maintenance or differentiation
Recombinant Human LIGHT/TNFSF14 Protein
By Anonymous on 11/16/2020
Application: Cell Adhesion