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Recombinant Human LILRA3/CD85e Fc Chimera Protein, CF

Catalog #: 9517-T4 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9517-T4-100
Recombinant Human LILRA3/CD85e Fc Chimera Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LILRA3/CD85e Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Angiopoietin‑like Protein 7/ANGPTL7 (Catalog # 914-AN) is coated onto a microplate at 2 µg/mL, Recombinant Human LILRA3/CD85e Fc Chimera (Catalog # 9517-T4) binds with an ED50 of 7-42 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human LILRA3/CD85e protein
Human LILRA3/CD85e
(Gly24-Glu439)
Accession # Q8N6C8-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q8N6C8-1
N-terminal Sequence
Analysis
Gly24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
72 kDa
SDS-PAGE
84-98 kDa, reducing conditions

Product Datasheets

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9517-T4

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9517-T4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human LILRA3/CD85e Fc Chimera Protein Bioactivity View Larger

When Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) is coated onto a microplate at 2 µg/mL, Recombinant Human LILRA3/CD85e Fc Chimera (Catalog # 9517-T4) binds with an ED50 = 7-42 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRA3/CD85e

Human LILRA3, also known as Leukocyte Ig-like receptor 4 (LIR-4), CD85 antigen-like family member E, Immunogobulin-like transcript 6 (ILT-6) and monocyte inhibitory receptor HM43/HM31,  is a 70 kD Ig superfamily member that belongs to the leukocyte receptor complex/cluster. Mature LILRA3 is 416 amino acids (aa) in length and contains four C2-type Ig-like domains (1, 2). Unlike other LILR family members, LILRA3 is actively secreted (3). Alternative splicing generates additional isoforms with  one containing an extra 18 aa in the signal peptide and/or a 64 amino acid deletion in the C-terminal half of the second Ig-like domain (4). Human LILRA3 shares ~86% aa identity with Chimpanzee LILRA3. Human LILRA3 acts as a soluble receptor for class I MHC antigens. It binds both classical and non‑classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. It also binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes (5). R&D Systems in-house testing indicates that LILRA3 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (6).

References
  1. Arm, J.P. et.al. (1997). J. Immunol. 159:2342.
  2. Ryu, M. et. al. (2011) PLoS One. 6:e19245.
  3. Martin, A.M. et al. (2002) Trends Immunol. 23:81
  4. Samaridis, J. & M. Colonna (1997) Eur. J. Immunol. 27:660.
  5. Lee T.H. et. al. (2013). J. Biol. Chem. 288:32873.
  6. Zheng, J. et al. (2012) Nature 485:656.
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM and ITIM Domains), Member 3
Entrez Gene IDs
11026 (Human)
Alternate Names
CD85 antigen-like family member E; CD85e antigen; CD85e; HM31; HM43; ILT6; ILT-6; ILT6CD85e; Immunoglobulin-like transcript 6; leucocyte immunoglobulin-like receptor; Leukocyte immunoglobulin-like receptor 4; leukocyte immunoglobulin-like receptor A3; leukocyte immunoglobulin-like receptor subfamily A member 3; leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member3; LILRA3; LIR4; LIR4CD85E; LIR-4HM43e3; Monocyte inhibitory receptor HM43/HM31

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