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Recombinant Human LINGO-1 Protein, CF

Catalog #: 9737-LG Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9737-LG-050
Recombinant Human LINGO-1 Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LINGO-1 Protein, CF Summary

Product Specifications

Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human Nogo Receptor Fc Chimera is present at 2.5 μg/mL, the concentration of Recombinant Human LINGO-1 that produces 50% of the optimal binding response is 0.4-2.4 μg/mL.  
Source
Human embryonic kidney cell, HEK293-derived human LINGO-1 protein
Thr40-Thr556, with a C-terminal 6-His tag
Accession #
Q96FE5-1
N-terminal Sequence
Analysis
Thr40
Predicted Molecular Mass
60 kDa
SDS-PAGE
82-112 kDa, reducing conditions

Product Datasheets

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9737-LG

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9737-LG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human LINGO-1 Protein Binding Activity View Larger

When Biotinylated Recombinant Human Nogo Receptor Fc Chimera is present at 2.5 μg/mL, Recombinant Human LINGO-1 (Catalog # 9737-LG) binds with an ED50 of 0.4-2.4 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LINGO-1

LINGO-1 (Leucine-rich repeat and immunoglobulin-like domain-containing Nogo receptor-interacting protein 1), also known as LERN1 (Leucin-rich repeat neuronal protein 1) or LRRN6A (Leucine-rich repeat neuronal protein 6A), is a 614 amino acid (aa) transmembrane protein of the leucine-rich repeat (LRR) family, ribonuclease inhibitor subfamily (1). The four known LINGO proteins (LINGO-1, -2, -3 and -4) contain LRR and IgCAM domains in the extracellular portion and share 44‑61% aa identity (2). LINGO-1 is restricted to the nervous system and is concentrated in the brain as a component of the NgR1/p75 and NgR1/Taj (TROY) signaling complexes (3). LINGO-1 negatively regulates neurite outgrowth and myelination. LINGO-1 is highly conserved, showing 99% aa identity between human, mouse and rat (2, 4). LINGO-1 promotes lysosomal degradation of amyloid-beta protein precursor and acts as a key player in Alzheimer's Disease progression (5). It suppresses oligodendrocyte precursor cell and myelin maturation, which leads to neurodegeneration (6). The intracellular LINGO-1 negatively regulates Trk receptors' signaling by modulating their lysosomal degradation (7). Inhibition of LINGO-1 may protect against degeneration of dopamine neurons in Parkinson's disease (8).

References
  1. Mosyak, L. et al. (2006) J. Biol. Chem. 281:36378.
  2. Mi, S. et al. (2004) Nat. Neurosci. 7:221.
  3. Fernandez-Enright, F. et al. (2014) Transl. Psychiatry. 4:e348.
  4. Mi, S. (2008) Cytokine Growth Factor Rev. 19:245.
  5. de Laat, R. et al. (2015) Pathobiol. Aging Age Relat. Dis. 5:25796.
  6. Foale, S. et al. (2017) Neural. Regen. Res. 12:1247.
  7. Meabon, J.S. et al. (2016) Mol. Cell Neurosci. 70:1.
  8. Inoue, H. et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104:14430.
Long Name
Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 1
Entrez Gene IDs
84894 (Human)
Alternate Names
FLJ14594; LERN1; LERN1UNQ201; leucine rich repeat and Ig domain containing 1; leucine rich repeat neuronal 6A; Leucine-rich repeat and immunoglobilin domain-containing protein 1; Leucine-rich repeat neuronal protein 1; Leucine-rich repeat neuronal protein 6A; LINGO1; LINGO-1; LRRN6A; LRRN6Aleucine-rich repeat neuronal 6A; MGC17422

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