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Recombinant Human LRP-1 Cluster IV Fc Chimera Protein, CF

Catalog #: 5395-L4B
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5395-L4B-050
Recombinant Human LRP‑1 Cluster IV Fc Chimera Protein Binding Activity.
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Product Details
Citations (6)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LRP-1 Cluster IV Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LRP-1 Cluster IV Fc Chimera (Catalog # 5395-L4B) is immobilized at 50 ng/mL (100 µL/well), Recombinant Human LRPAP (Catalog # 4296-LR) binds with an ED50 of 0.0250-0.350 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human LRP-1 Cluster IV protein
Human LRP1-C4
(Ser3332-Asp3779)
Accession # Q07954.2		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
Q07954.2
N-terminal Sequence
Analysis
Ser3332
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
76.7 kDa
SDS-PAGE
100-120 kDa, under reducing conditions.

Product Datasheets

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5395-L4B

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5395-L4B

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human LRP‑1 Cluster IV Fc Chimera (Catalog # 5395-L4B) is immobilized at 50 ng/mL (100 µL/well), Recombinant Human LRPAP (4296-LR) binds with an ED50 of 0.0250-0.350 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human LRP-1 Cluster IV Fc Chimera Protein (Catalog # 5395-L4B) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-120 kDa and 200-240 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRP-1 Cluster IV

LDL receptor-related protein 1 (LRP-1), also known as CD91 and the alpha 2-macroglobulin receptor, is a type I membrane protein in the LDL receptor superfamily. It is expressed on neurons, hepatocytes, adipocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. LRP-1 is important for the clearance of a large number of circulating molecules involved in fatty acid metabolism and complexes of serine proteases with their inhibitors (1-4). LRP-1 also associates directly or through intracellular scaffold proteins with other membrane associated proteins on the same cell. This allows LRP-1 to modulate the activity or internalization of PDGF R beta, NMDA receptor subunits, TGF-beta receptors, Frizzled-1, various integrins, and the prion protein PrPc. Human LRP-1 is an N-glycosylated and sialylated molecule that is cleaved in the Golgi to produce an 85 kDa transmembrane beta  chain and a 515 kDa alpha  chain that associates noncovalently with the beta  chain but does not itself cross the membrane (11, 12). The alpha chain of LRP-1 contains 31 LDLR class A repeats, 34 LDLR class B repeats, and 22 EGF-like repeats (13). The LDLR domains are clustered in four regions throughout the protein (13). Cluster IV (aa 3332-3779) contains eleven LDLR class A repeats (14). Within this region, human LRP-1 shares 99% aa sequence identity with mouse and rat LRP-1. A shed soluble form of LRP-1 circulates in the serum and retains ligand binding properties (15). Cluster IV contains binding sites for Apolipoprotein E, LPL, and LRPAP/RAP,  alpha 2-macroglobulin, Coagulation Factor VIII light chain, Lactoferrin, PAI-1, tPA-PAI-1 complexes, Pro-uPA, and TFPI (14).

References
  1. Lillis, A.P. et al. (2008) Physiol. Rev. 88:887.
  2. Galliano, M.-F. et al. (2008) PloS ONE 3:e2729.
  3. Bouchard, B.A. et al. (2007) J. Thromb. Haemost. 6:638.
  4. Sendra, J. et al. (2008) Cardiovasc. Res. 78:581.
  5. Takayama, Y. et al. (2005) J. Biol. Chem. 280:18504.
  6. Martin, A.M. et al. (2008) J. Biol. Chem. 283:12004.
  7. Cabello-Verugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
  8. Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
  9. Taylor, D.R. and N.M. Hooper (2007) Biochem. J. 402:17.
  10. Parkyn, C.J. et al. (2007) J. Cell Sci. 121:773.
  11. Herz, J. et al. (1990) EMBO J. 9:1769.
  12. Strickland, D.K. et al. (1990) J. Biol. Chem. 265:17401.
  13. Herz, J. et al. (1988) EMBO J. 7:4119.
  14. Neels, J.G. et al. (1999) J. Biol. Chem. 274:31305.
  15. Quinn, K.A. et al. (1999) Exp. Cell Res. 251:433.
Long Name
LDL Receptor-related Protein 1, Cluster II
Entrez Gene IDs
4035 (Human); 16971 (Mouse); 299858 (Rat)
Alternate Names
LRP1 Cluster IV; LRP-1 Cluster IV

Citations for Recombinant Human LRP-1 Cluster IV Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Generation of KS-487 as a novel LRP1-binding cyclic peptide with higher affinity, higher stability and BBB permeability
    Authors: K Sakamoto
    Biochemistry and Biophysics Reports, 2022-10-08;32(0):101367.
    Species: N/A
    Sample Types: Peptide
    Applications: ELISA Capture
  2. A novel LRP1-binding peptide L57 that crosses the blood brain barrier
    Authors: K Sakamoto, T Shinohara, Y Adachi, T Asami, T Ohtaki
    Biochem Biophys Rep, 2017-08-12;12(0):135-139.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Evidence that factor VIII forms a bivalent complex with the LDL receptor-related protein 1 (LRP1): Identification of cluster IV on LRP1 as the major binding site
    Authors: Dudley K Strickland
    J. Biol. Chem., 2016-10-29;0(0):.
    Applications: Bioassay
  4. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities.
    Authors: Thevenard J, Verzeaux L, Devy J, Etique N, Jeanne A, Schneider C, Hachet C, Ferracci G, David M, Martiny L, Charpentier E, Khrestchatisky M, Rivera S, Dedieu S, Emonard H
    PLoS ONE, 2014-07-30;9(7):e103839.
    Applications: Bioassay
  5. LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins.
    Authors: Schorch B, Song S, van Diemen F, Bock H, May P, Herz J, Brummelkamp T, Papatheodorou P, Aktories K
    Proc Natl Acad Sci U S A, 2014-04-15;111(17):6431-6.
    Applications: Enzyme Assay
  6. A small molecule inhibitor to plasminogen activator inhibitor 1 inhibits macrophage migration.
    Authors: Ichimura A, Matsumoto S, Suzuki S, Dan T, Yamaki S, Sato Y, Kiyomoto H, Ishii N, Okada K, Matsuo O, Hou F, Vaughan D, van Ypersele de Strihou C, Miyata T
    Arterioscler Thromb Vasc Biol, 2013-03-07;33(5):935-42.
    Applications: Bioassay

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