Recombinant Human LRRTM2 Fc Chimera Protein, CF

Catalog #: 5589-LR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
5589-LR-050

Recombinant Human LRRTM2 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LRRTM2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat Neurexin 1 alpha (-S4) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human LRRTM2 Fc Chimera that produces 50% of the optimal binding response is 0.07-0.42 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human LRRTM2 protein

Human LRRTM2 (Cys34-Arg422) Accession # O43300	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

O43300

N-terminal Sequence
Analysis

Cys34

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

71 kDa

SDS-PAGE

83-100 kDa, reducing conditions

Product Datasheets

5589-LR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5589-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Rat Neurexin 1 alpha (-S4) is coated at 1 μg/mL (100 μL/well), Recombinant Human LRRTM-2 Fc Chimera (Catalog # 5589-LR) binds with an ED₅₀ of 0.07-0.42 μg/mL

SDS-PAGE

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2 μg/lane of Recombinant Human LRRTM2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-100 kDa and 160-200 kDa, respectively.

Reconstitution Calculator

Background: LRRTM2

LRRTM2 (Leucine-rich repeat transmembrane protein 2) is a member of the LRRTM family of molecules (1). All LRRTMs are type I transmembrane proteins that contain multiple leucine rich repeats and one PDZ consensus cytoplasmic binding domain. The LRRTM family is expressed in the central nervous system across vertebrate species, and they are not found in invertebrates (1). Human LRRTM2 is synthesized as a 516 amino acid (aa) precursor that contains a 33 aa signal sequence, a 389 aa extracellular domain, a 21 aa transmembrane segment, and a 73 aa cytoplasmic region (1). The extracellular domain is characterized by the presence of ten Leucine-rich repeats, flanked by two cysteine-rich sequences. Mature human LRRTM2 is 98% aa identical to mouse LRRTM2 (1). LRRTM2 functions as a postsynaptic organizer in excitatory synapses. LRRTM2 binds only Neurexin-alpha and Neurexin-beta which are lacking splice site 4 (S4) (2). Crystal structure shows dependence of this interaction on Calcium ion as well as overlapping binding interface with Neuroligins (3). LRRTM2 is essential for long term potentiation in hippocampal neuron by maintaining AMPA Receptors at the synapse (4).

References