Recombinant Human Ly-6G6F Fc Chimera Protein, CF

Recombinant Human Ly-6G6F Fc Chimera Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Ly-6G6F

Lymphocyte antigen 6 complex locus protein G6f, also known as G6f, is a 32-kDa single pass type I membrane protein belonging to the Immunoglobulin Superfamily that is encoded in the MHC (1). The extracellular domain is 281 aa in length and contains one disulfide bond (C35 ↔ C106) and an N-linked glycosylation site at S88. The ECD of human Ly-6G6F shares 64.5% and 63% sequence identity with mouse and rat Ly-6G6F, respectively. The intracellular tail of Ly-6G6F is 40 amino acids in length and contains one tyrosine residue (Y281), which is phosphorylated after treatment of cells with pervanadate. Ly-6G6F may play a role in signal transduction pathways involved with Grb2 and Grb7, such as the Ras-MAP kinase pathway (1). Ly-6G6F tyrosine phosphorylation was also found to take place in response to collagen, although not in response to the G protein-coupled receptor agonists, thrombin and ADP. A global proteomics approach identifies Ly-6G6F as a novel phosphorylated signaling proteins in GPVI-activated platelets (2, 3).  Leukocytes mRNA levels of Ly-6G6F mainly involved in inflammation were significantly higher in patients carrying granulin mutations compared with asymptomatic carriers (4).