Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein, CF

Catalog #: AVI8884 Datasheet / COA / SDS

Biotinylated His-tag

Catalog #	Availability	Size / Price	Qty
AVI8884-025

Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Lymphotoxin beta R Fc Chimera (Catalog # 629-LR) is immobilized at 25 ng/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Lymphotoxin alpha1/beta2 His-tag Avi-tag (Catalog # AVI8884) that produces 50% of the optimal binding response is 0.75-4.5 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human Lymphotoxin protein

Human Lymphotoxin alpha (Leu35-Leu205) Accession # P01374	GGGGS	Human Lymphotoxin beta (Gln49-Gly244) Accession # Q06643-1	GGGGS	Human Lymphotoxin beta (Gln49-Gly244) Accession # Q06643-1	HHHHHH, Avi-tag
N-terminus

Accession #

P01374 (alpha) & Q06643-1 (beta)

N-terminal Sequence
Analysis

Leu35

Structure / Form

GS-linked heterotrimer, biotinylated via Avi-tag

Predicted Molecular Mass

63 kDa

SDS-PAGE

69-80 kDa

Product Datasheets

AVI8884

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI8884

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Lymphotoxin beta R/TNFRSF3 Fc Chimera (Catalog # 629-LR) is immobilized at 25 ng/mL, 100 µg/well, Biotinylated Recombinant Human Lymphotoxin alpha1/beta2 His-tag Avi-tag (Catalog # AVI8884) binds with an ED₅₀ of 0.75-4.5 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Lymphotoxin alpha1/beta2 His-tag Avi-tag (Catalog # AVI8884) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 69-80 kDa.

Reconstitution Calculator

Background: Lymphotoxin

Lymphotoxin alpha (LT-alpha ), and Lymphotoxin beta (LT-beta ) are pro-inflammatory TNF superfamily ligands that play important roles in immune system development (1, 2). The 25 kDa mature human LT-alpha is a secreted protein that shares 75% amino acid (aa) sequence identity with mouse and rat LT-alpha (3, 4). The 33 kDa mature human LT-beta is a type II transmembrane protein that shares 73% aa sequence identity with mouse and rat LT-beta within common regions of their extracellular domains (5). Relative to the human protein, mouse and rat LT-beta have a 66 aa or 65 aa insertion within the ECD, respectively. LT-alpha can be secreted as a homotrimer that binds and activates TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, HVEM/TNFRSF14, and Troy/TNFRSF19 (6-8). LT-alpha is required for development of the spleen, lymph nodes, and Peyer’s patches (9). It also regulates T cell homing to the gut and IgA induction (10). In addition, LT-alpha can form membrane-associated heterotrimers with two copies of LT-beta on the surface of B, T, LTi, and ILC3 cells (2, 5, 11). The Lymphotoxin alpha 1/ beta 2 heterotrimer binds and activates the Lymphotoxin beta R/TNFRSF3 (LT beta R) which is expressed on macrophages, dendritic cells, hepatocytes, intestinal epithelial cells (IEC), follicular dendritic cells (FDC), and high endothelial venules (HEV) (2, 12, 13). LT beta R also serves as a receptor for LIGHT/TNFSF14 (14). LT-alpha 1/ beta 2 promotes the development of FDC networks and HEV in lymphoid tissue, the class switching of immature B cells for IgA production, and the production of homeostatic IL-22 by ILCs (10, 15-17). It can be shed by ADAM17 or MMP-8 mediated cleavage, and the released heterotrimer circulates in the serum and is elevated in synovial fluid of rheumatoid arthritis patients (18).

References