Recombinant Human Lymphotoxin alpha2/beta1 Protein Summary
Product Specifications
Leu35-Leu205 (LT alpha ) & Leu54-Gly244 (LT beta ), both with an N-terminal Met & Thr41 ‑ Leu205 (LT alpha )
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
679-TX
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
679-TX/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Lymphotoxin
Lymphotoxin-alpha (LT-alpha ), also known as tumor necrosis factor (TNF) beta, and lymphotoxin-beta (LT-beta ) are members of the TNF family. LT-alpha cDNA encodes a 205 amino acid secreted soluble protein with a 34 amino acid signal sequence. LT-beta cDNA encodes a 244 amino acid type II membrane protein with an N-terminal cytoplasmic domain (residues 1 - 18), a transmembrane region (residues 19 - 48), and an extracellular domain (residues 49 - 244). Secreted LT-alpha assembles as a soluble homotrimer, LT-alpha 3. In addition, secreted LT-alpha also complexes with the membrane associated LT-beta to generate two types of heterotrimers, LT-alpha 1/ beta 2 and LT-alpha 2/ beta 1 (1). The soluble LT-alpha 3 binds both TNF RI (p55) and TNF RII (p75). In contrast, the predominant membrane-bound heterotrimer, LT-alpha 1/ beta 2, binds only to the lymphotoxin beta receptor (LT beta R). LT-alpha 2/ beta 1 is capable of binding LT beta R, TNF RI (p55), and TNF RII (p75). LT plays a role in normal lymphoid organogenesis (2, 3). Transgenic LT-alpha knock-out mice exhibited a loss in lymph node development, a change in splenic architecture, and impaired germinal center formation (4). LT is expressed by activated naive CD4 cells, unpolarized IL-2-secreting effectors, and Th1 effectors. A loss of LT expression and lack of TNF-alpha or TNF-beta secretion is associated with prior exposure to IL-4 and a Th2 phenotype (5).
- Browning et al. (1993) Cell 72:847.
- Ettinger et al. (1996) PNAS 93:13102.
- Cuff et al. (1998) J. Immunol. 161:6853.
- Browning et al. (1997) J. Immunol. 159:3288.
- Gramaglia et al. (1999) J. Immunol. 162:1333.
Citations for Recombinant Human Lymphotoxin alpha2/beta1 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
MAbs, 2018-03-05;0(0):1-43.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
Chronic lung injury by constitutive expression of activation-induced cytidine deaminase leads to focal mucous cell metaplasia and cancer.
Authors: Kitamura, Jiro, Uemura, Munehiro, Kurozumi, Mafumi, Sonobe, Makoto, Manabe, Toshiaki, Hiai, Hiroshi, Date, Hiroshi, Kinoshita, Kazuo
PLoS ONE, 2015-02-06;10(2):e0117986.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
LIGHT, a member of the tumor necrosis factor ligand superfamily, prevents tumor necrosis factor-alpha-mediated human primary hepatocyte apoptosis, but not Fas-mediated apoptosis.
Authors: Hikichi Y, Tsuji I, Shintani Y
J. Biol. Chem., 2002-10-18;277(51):50054-61.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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