Recombinant Human M-Cadherin/Cadherin-15 Fc Chimera, CF
Recombinant Human M-Cadherin/Cadherin-15 Fc Chimera, CF Summary
Product Specifications
Optimal concentration depends on cell type as well as the application or research objectives.
| Human M-CAD (Val22 - Ala606) Accession # P55291 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4096-MC
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl2. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: M-Cadherin/Cadherin-15
M-Cadherin (M-CAD or Cadherin-15) is a 124 kDa type I transmembrane glycoprotein of the Cadherin superfamily of calcium-dependent homotypic adhesion molecules (1 - 4). Like other classical Cadherins, the 814 amino acid (aa) human M-CAD contains a signal sequence (21 aa), a propeptide (29 aa), an extracellular domain with five Cadherin domain repeats (ECD, 556 aa), a transmembrane segment (20 aa) and a cytoplasmic domain (188 aa) (1). A splice variant that diverges within the third Cadherin repeat has been sequenced. The Cadherin repeats are responsible for cell-cell adhesion by homophilic binding on opposing cells (1 - 4). Intracellularly, M-CAD binds beta -catenin or plakoglobin ( gamma -catenin), which in turn bind alpha -catenin (4). M-CAD also binds p120 catenin (5). Connection of Cadherin/catenin complexes to the actin cytoskeleton is in question, but is possible through a linker (6). Connection to microtubules has been shown (7). M-CAD is present during early stages of skeletal muscle development and is thought to align myoblasts for fusion (8). It is also present in muscle satellite cells and participates in muscle regeneration (9). M-CAD is also expressed in the granule cell layer of the cerebellar glomerulus (10). Deletion of mouse M-CAD has little effect in vivo, most likely due to compensation by N-Cadherin (11). However, M-CAD upregulation and adhesion between myoblasts during induction of differentiation in vitro is required for their fusion (8, 12, 13). M-CAD activity is later downregulated by sequestering to caveoli, p120 catenin/RhoA-induced ubiquitination and/or cleavage by calpain-3. This terminates fusion and allows sarcomere formation (5, 12, 13). Human M-CAD ECD shows 88% aa identity with mouse, rat or bovine, and 85% aa identity with canine M-CAD ECD. M-CAD is an outlier among classical Cadherins, with 40% aa identity or less in the ECD (4).
- Kaufmann, U. et al. (1999) Cell Tissue Res. 296:191.
- Angst, B. D. et al. (2001) J. Cell Sci. 114:629.
- Shimoyama, Y. et al. (1998) J. Biol. Chem. 273:10011.
- Kuch, C. et al. (1997) Exp. Cell Res. 232:331.
- Charasse, S. et al. (2006) Mol. Biol. Cell 17:749.
- Weis, W. I. and W. J. Nelson (2006) J. Biol. Chem. 281:35593.
- Kaufmann, U. et al. (1999) J. Cell Sci. 112:55.
- Zeschnigk, M. et al. (1995) J. Cell Sci. 108:2973.
- Wrobel, E. et al. (2007) Eur. J. Cell Biol. Jan 10; [Epub ahead of print]
- Rose, O. et al. (1995) Proc. Natl. Acad. Sci. USA 92:6022.
- Hollnagel, A. et al. (2002) Mol. Cell. Biol. 22:4760.
- Volonte, D. et al. (2003) Mol. Biol. Cell 14:4075.
- Kramerova, I. et al. (2006) Mol. Cell. Biol. 26:8437.
Citation for Recombinant Human M-Cadherin/Cadherin-15 Fc Chimera, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Homeostatic regulation of T cell trafficking by a B cell-derived peptide is impaired in autoimmune and chronic inflammatory disease.
Authors: Chimen M, McGettrick H, Apta B, Kuravi S, Yates C, Kennedy A, Odedra A, Alassiri M, Harrison M, Martin A, Barone F, Nayar S, Hitchcock J, Cunningham A, Raza K, Filer A, Copland D, Dick A, Robinson J, Kalia N, Walker L, Buckley C, Nash G, Narendran P, Rainger G
Nat Med, 2015-04-20;21(5):467-75.
Species: Human
Sample Types: Protein
Applications: Bioassay
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