Recombinant Human Matrilin-2 Protein Summary
Product Specifications
Arg24-Arg937, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3044-MN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
3044-MN/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Matrilin-2
Matrilin-2 is an extracellular matrix protein that belongs to the superfamily of von Willebrand factor A (VWA) containing proteins. It is expressed in many tissues and functions as a bridging component between other matrix proteins (1 - 4). The human Matrilin-2 cDNA encodes a 956 amino acid (aa) precursor with a 23 aa signal sequence, two VWA domains separated by ten tandem EGF-like repeats, and a C-terminal coiled-coil domain (5, 6). Alternate splicing generates Isoform 2 (with an 18 aa deletion near the C-terminus), Isoform 3 (with a deletion of the fourth EGF-like repeat), and Isoform 4 (with a deletion of the first VWA and first EGF-like repeat). Human Matrilin-2 shares 87% and 84% aa sequence identity with mouse and canine Matrilin-2, respectively, and 27%, 22%, and 33% aa sequence identity with human Matrilin-1, -3, and -4, respectively. Matrilin-2 forms a variety of disulfide-linked oligomers via its coiled-coil domain (4, 7, 8, 9). It can assemble into homotrimers or heterotrimers with Matrilin-1 and/or Matrilin-4 (4, 7, 8) but has not been detected in heterotrimers containing Matrilin-3 (8). The VWA domains are thought to mediate Matrilin-Matrilin interactions as well as interactions with other matrix proteins such as Fibronectin, Collagen I, Fibrillin-2, and Laminin-1/Nidogen-1 complexes (7). Matrilin-2 knockout mice do not display any obvious abnormalities, suggesting that the expression of other molecules can compensate for the lack of Matrilin-2 (10).
- Wagener, R. et al. (2005) FEBS Lett. 579:3323.
- Deak, F. et al. (1999) Matrix Biol. 18:55.
- Whittaker, C.A. and R.O. Hynes (2002) Mol. Biol. Cell 13:3369.
- Piecha, D. et al. (1999) J. Biol. Chem. 274:13353.
- Muratoglu, S. et al. (2000) Cytogenet. Cell Genet. 90:323.
- Deak, F. et al. (1997) J. Biol. Chem. 272:9268.
- Piecha, D. et al. (2002) Biochem. J. 367:715.
- Frank, S. et al. (2002) J. Biol. Chem. 277:19071.
- Pan, O.H. and K. Beck (1998) J. Biol. Chem. 273:14205.
- Mates, L. et al. (2004) Matrix Biol. 23:195.
Citations for Recombinant Human Matrilin-2 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Interleukin 38 alleviates aortic valve calcification by inhibition of NLRP3
Authors: E The, DM de Graaf, Y Zhai, Q Yao, L Ao, DA Fullerton, CA Dinarello, X Meng
Proceedings of the National Academy of Sciences of the United States of America, 2022-08-29;119(36):e2202577119.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells
Authors: E The, Q Yao, P Zhang, Y Zhai, L Ao, DA Fullerton, X Meng
Cells, 2020-02-07;9(2):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells
Authors: F Li, R Song, L Ao, TB Reece, JC Cleveland, N Dong, DA Fullerton, X Meng
Arterioscler. Thromb. Vasc. Biol., 2017-05-25;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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