Recombinant Human MEGF9 His-tag Protein, CF Summary
Product Specifications
Ala36-Asn514, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10514-ME
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 1 mg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Human MEGF9 His-tag (Catalog # 10514-ME) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 136-150 kDa.
Reconstitution Calculator
Background: MEGF9
MEGF9 (Multiple EGF‑like domains protein 9; also EGF‑like protein 5) is a novel transmembrane glycoprotein with multiple EGF-like repeats. It is predominantly expressed in the developing and adult central nervous system and peripheral nervous system. Immunohistochemical studies showed that MEGF9 is expressed in Purkinje cells of the cerebellum and in glial cells of the peripheral nervous system. In non-neuronal tissues, MEGF9 is expressed in epidermal layer of skin, papillae of the tongue and epithelium of the gastrointestinal tract. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration. Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular domain (ECD). The extracellular region possesses a lengthy Pro‑rich region (aa 55‑200). N-terminal region of MEGF9 includes several potential O-glycosylation sites followed by five EGF-like domains which are highly homologous to the short arms of laminins. Following one single pass transmembrane domain, a highly conserved short intracellular domain with potential phosphorylation sites is present (1, 2). Within the ECD, human MEGF9 shares 75% aa sequence identity with mouse and rat MEGF9.
- Brandt-Bohne, U. et al. (2007) Biochem. J. 401:447.
- Heller, B. et al. (2014) J. Cell. Biol. 204:1219.
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