Recombinant Human MFAP3 Fc Chimera Protein, CF

Catalog #: 10800-MF Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10800-MF-050

Recombinant Human MFAP3 Fc Chimera Protein SDS-PAGE.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human MFAP3 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human MFAP3 Fc Chimera, immobilized at 2.5-5.0 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.

Source

Human embryonic kidney cell, HEK293-derived human MFAP3 protein

Human MFAP3 (Leu22-Met147) Accession # P55082.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

P555082.1

N-terminal Sequence
Analysis

Leu22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

41 kDa

SDS-PAGE

57-67 kDa, under reducing conditions

Product Datasheets

10800-MF

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10800-MF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human MFAP3 Fc Chimera (Catalog # 10800-MF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-67 kDa and 114-134 kDa, respectively.

Reconstitution Calculator

Background: MFAP3

Microfibril-associated glycoprotein 3 (MFAP3), is a member of the MFAP family which play a role in microfibril assembly, elastinogenesis, and tissue homeostasis. The family members were identified as potential components of fibrillin-containing microfibrils, but they do not share structural similarities (1). MFAP3 is found in ocular zonules. Human MFAP3 is a serine-rich acidic protein located in zonular fibers and consists of an extracellular domain (ECD) containing one Ig-like C2-type domain, a single transmembrane domain, and a cytoplasmic domain. Within the ECD, mature human MFAP3 shares 74% amino acid sequence identity with mouse and rat MFAP3. Among MFAP3-related pathways are degradation of the extracellular matrix and elastic fiber formation. MFAP is a candidate gene for heritable diseases affecting microfibrils (2).

References