Recombinant Human MICA Fc Chimera Avi-tag Protein, CF

Catalog #: AVI1300 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI1300-050

Recombinant Human MICA Fc Chimera Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human MICA Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D Fc Chimera (Catalog # 1299-NK) is immobilized at 1 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human MICA Fc Chimera Avi-tag (Catalog # AVI1300) that produces 50% of the optimal binding response is found to be approximately 7.5-45 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human MICA protein

Human MICA
(Ala23-Gln308)
Accession # NP_001170990.1

IEGRMD

Human IgG₁
(Pro100-Lys330)

Avi-tag

Accession #

NP_001170990.1

N-terminal Sequence
Analysis

Ala23

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Predicted Molecular Mass

61 kDa

SDS-PAGE

85-100 kDa, under reducing conditions

Product Datasheets

AVI1300

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI1300

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human NKG2D Fc Chimera (Catalog # 1299-NK) is immobilized at 1 µg/mL (100 µL/well), the concentration of Biotinylated Human MICA Fc Chimera Avi-tag (Catalog # AVI1300) that produces 50% of the optimal binding response is found to be approximately 7.5-45 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human MICA Fc Chimera Avi-tag (Catalog # AVI1300) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-100 kDa and 170-200 kDa, respectively.

Reconstitution Calculator

Background: MICA

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

References