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Recombinant Human MICA Fc Chimera Protein, CF

Catalog #: 1300-MA
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1300-MA-050
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Citations (11)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human MICA Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind rhNKG2D/Fc Chimera in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived human MICA protein
Human MICA
(Ala23 - Gln308)
Accession # NP_001170990		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
NP_001170990
N-terminal Sequence
Analysis
Ala23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
59 kDa (monomer)
SDS-PAGE
90-100 kDa, reducing conditions

Product Datasheets

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1300-MA

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1300-MA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MICA

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

References
  1. Groh, V. et al. (2001) Nature Immunol. 2:255.
  2. Stephens, H. (2001) Trends Immunol. 22:378.
  3. Bauer, S. et al. (1999) Science 285:727.
  4. Groh, V. et al. (2002) Nature 419:734.
  5. Steinle, A. et al. (2001) Immunogenetics 53:279.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. NKG2D and its Ligands (2002) http://www.RnDSystems.com
Long Name
MHC Class I-related Protein A
Entrez Gene IDs
100507436 (Human)
Alternate Names
FLJ60820; MGC111087; MICA; PERB11.1

Citations for Recombinant Human MICA Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Soluble NKG2DLs Are Elevated in Breast Cancer Patients and Associate with Disease Outcome
    Authors: Seller, A;Tegeler, CM;Mauermann, J;Schreiber, T;Hagelstein, I;Liebel, K;Koch, A;Heitmann, JS;Greiner, SM;Hayn, C;Dannehl, D;Engler, T;Hartkopf, AD;Hahn, M;Brucker, SY;Salih, HR;Märklin, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
    Applications: ELISA Standard
  2. Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion
    Authors: JA Myers, D Schirm, L Bendzick, R Hopps, C Selleck, P Hinderlie, M Felices, JS Miller
    JCI Insight, 2022-08-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. An actin cytoskeletal barrier inhibits lytic granule release from natural killer cells in patients with Chediak-Higashi syndrome
    Authors: A Gil-Krzews, MB Saeed, A Oszmiana, ER Fischer, K Lagrue, WA Gahl, WJ Introne, JE Coligan, DM Davis, K Krzewski
    J. Allergy Clin. Immunol., 2017-12-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition
    Authors: AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr
    Oncotarget, 2017-05-09;8(19):31959-31976.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Standard)
  5. Lenalidomide augments actin remodeling and lowers NK-cell activation thresholds.
    Authors: Lagrue K, Carisey A, Morgan D, Chopra R, Davis D
    Blood, 2015-05-22;126(1):50-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Super-resolution imaging of remodeled synaptic actin reveals different synergies between NK cell receptors and integrins.
    Authors: Brown A, Dobbie I, Alakoskela J, Davis I, Davis D
    Blood, 2012-09-10;120(18):3729-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Selective induction of host genes by MVA-B, a candidate vaccine against HIV/AIDS.
    Authors: Guerra S, Gonzalez JM, Climent N, Reyburn H, Lopez-Fernandez LA, Najera JL, Gomez CE, Garcia F, Gatell JM, Gallart T, Esteban M
    J. Virol., 2010-06-09;84(16):8141-52.
    Applications: ELISA (Standard)
  8. The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia.
    Authors: Nuckel H, Switala M, Sellmann L, Horn PA, Durig J, Duhrsen U, Kuppers R, Grosse-Wilde H, Rebmann V
    Leukemia, 2010-04-29;24(6):1152-9.
    Applications: ELISA (Standard)
  9. Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity.
    Authors: Kwong KY, Baskar S, Zhang H, Mackall CL, Rader C
    J. Mol. Biol., 2008-09-16;384(5):1143-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. NKG2D ligand expression in AML increases in response to HDAC inhibitor valproic acid and contributes to allorecognition by NK-cell lines with single KIR-HLA class I specificities.
    Authors: Diermayr S, Himmelreich H, Durovic B, Mathys-Schneeberger A, Siegler U, Langenkamp U, Hofsteenge J, Gratwohl A, Tichelli A, Paluszewska M, Wiktor-Jedrzejczak W, Kalberer CP, Wodnar-Filipowicz A
    Blood, 2007-11-09;111(3):1428-36.
    Applications: ELISA (Standard)
  11. Human cytomegalovirus-encoded UL16 discriminates MIC molecules by their alpha2 domains.
    Authors: Spreu J, Stehle T, Steinle A
    J. Immunol., 2006-09-01;177(5):3143-9.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay

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