Home / MINDY2 / Recombinant Human MINDY2/FAM63B Protein, CF

Recombinant Human MINDY2/FAM63B Protein, CF

Catalog #: E-620 Datasheet / COA / SDS

Discontinued Product

E-620 has been discontinued.
View all MINDY2 products.
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human MINDY2/FAM63B Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human MINDY-2 is a Ubiquitin-specific deconjugating enzyme that cleaves K48-linked poly-Ubiquitin chains.
Source
E. coli-derived human MINDY2 protein
Accession #
Q8NBR6
Predicted Molecular Mass
67 kDa
SDS-PAGE

Product Datasheets

You must select a language.

x

E-620

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-620

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: MINDY2

MINDY-2/FAM63B is a member of a newly characterized cysteine protease family of deubiquitinases ("DUBs").  In humans, the MINDY (MIU containing novel DUB family) family has at least four members, with an additional three being MINDY-1/FAM63A, MINDY-3/FAM188A and MINDY-4/FAM188B.  The catalytic domain of MINDY DUBs adopts a conformation that is distinct from other known DUB classes, and these enzymes are highly selective at cleaving K48-linked polyubiquitin chains.  With a predicted molecular weight of 67 kDa, human MINDY-2 is 82% identical to both its murine and rat orthologs.  This recombinant protein is untagged.

References
  1. Abdul-Rehman S.A. et al. (2016)  Mol. Cell 63: 146
  2. Kristariyanto Y.A. et al. (2017) EMBO Reports  DOI 10.15252/embr.201643205

Long Name
Ubiquitin Carboxyl-Terminal Hydrolase MINDY-2
Entrez Gene IDs
54629 (Human); 235461 (Mouse); 363089 (Rat)
Alternate Names
FAM63B family with sequence similarity 63, member B; FAM63B; MINDY2; MINDY-2

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human MINDY2/FAM63B Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human MINDY2/FAM63B Protein, CF and earn rewards!

Have you used Recombinant Human MINDY2/FAM63B Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review