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Recombinant Human/Mouse Frizzled-2 Fc Chimera Protein, CF

Catalog #: 1307-FZ
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1307-FZ-050
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Citations (5)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human/Mouse Frizzled-2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind biotinylated Wnt-5a in a functional ELISA with an estimated Kd <5 nM.
Source
Mouse myeloma cell line, NS0-derived Frizzled-2 protein
Human/Mouse Frizzled-2
(Gln29-Leu168)
Accession # Q9JIP6		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q9JIP6
N-terminal Sequence
Analysis
No results obtained: Gln29 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
42.3 kDa (monomer)
SDS-PAGE
50-55 kDa, reducing conditions

Product Datasheets

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1307-FZ

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1307-FZ

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Frizzled-2

Wnt signaling is involved in variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The founding member of this family was identified in Drosophila based on its role in tissue polarity in the adult cuticle and named for the disorganized appearance of bristle hairs on the mutant. Ten mammalian Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. One of the most conserved regions of the Frizzled proteins is the extracellular cysteine-rich domain (CRD) which spans approximately 120 amino acids (aa) and contains 10 invariant cysteines (1). Human and mouse Frizzled-2 are 100% identical at the aa level. Frizzled-2 expression is greater in embryonic than adult tissues, with heart, brain, lung, kidney and gut showing the highest levels (2). In addition, Frizzled-2 is expressed during migration and proliferation of neural crest cells that populate the heart and great arteries (3). However, Frizzled-2 may not be strictly involved in developmental processes, as its expression is also up-regulated in myofibroblasts during tissue repair after myocardial infarction (4). Frizzled-2 is implicated in the Wnt/Ca2+ pathway, a mechanism by which Wnt-5a signaling results in calcium release from intracellular stores and activation of PKC and calmodulin-dependent protein kinase (5). Secreted frizzled related proteins (sFRPs), antagonists of the Wnt pathway, have been shown to interact with both Wnt ligands and Frizzled receptors (6).

References
  1. Wang, Y. et al. (1996) J. Biol. Chem 271:4468.
  2. Malik, T. and R. Shivdasani (2000) Biochem. J. 349:829.
  3. van Gijn, M. et al. (2001) Anat. Embryol. 203:185.
  4. Toyofuku, T. et al. (2000) J. Cell Biol. 150:225.
  5. Kuhl, M. et al. (2000) Trends Genet. 16: 279.
  6. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
Entrez Gene IDs
2535 (Human); 57265 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 2; frizzled homolog 2 (Drosophila); Frizzled2; Frizzled-2; Fz2; fz-2; FZD2; fzE2; hFz2

Citations for Recombinant Human/Mouse Frizzled-2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Identification of TFPI as a receptor reveals recombination-driven receptor switching in Clostridioides difficile toxin B variants
    Authors: S Tian, X Xiong, J Zeng, S Wang, BJ Tremblay, P Chen, B Chen, M Liu, P Chen, K Sheng, D Zeve, W Qi, DT Breault, C Rodríguez, R Gerhard, R Jin, AC Doxey, M Dong
    Nature Communications, 2022-11-09;13(1):6786.
    Species: Human
    Sample Types: Protein
    Applications: Reference Standard
  2. A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy
    Authors: R Chidiac, M Abedin, G Macleod, A Yang, PE Thibeault, LL Blazer, JJ Adams, L Zhang, H Roehrich, HN Jo, S Seshagiri, SS Sidhu, HJ Junge, S Angers
    Embo Molecular Medicine, 2021-06-09;0(0):e13977.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Designed Ankyrin Repeat Protein (DARPin) Neutralizers of TcdB from Clostridium difficile Ribotype 027
    Authors: Z Peng, R Simeon, SB Mitchell, J Zhang, H Feng, Z Chen
    mSphere, 2019-10-02;4(5):.
    Species: Human
    Sample Types: Protein
    Applications: ELISA (Capture)
  4. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
    J Biol Chem, 2016-07-11;0(0):.
    Applications: Bioassay
  5. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm.
    Authors: Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM
    Development, 2006-08-30;133(19):3787-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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