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Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF

Catalog #: E2-656
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
E2-656-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (7)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human UBE2M/Ubc12 is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2M/Ubc12 concentration of 0.1-1 μM.
Source
E. coli-derived UBE2M/Ubc12 protein
Met1 - Lys183
Accession #
P61081.1
Predicted Molecular Mass
21 kDa

Product Datasheets

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E2-656

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-656

Formulation Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2M/Ubc12

Ubiquitin-conjugating Enzyme E2M (UBE2M), also known as Ubiquitin-conjugating Enzyme 12 (Ubc12), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. UBE2M/Ubc12 is highly conserved, and the human ortholog shares 100% amino acid identity with mouse and rat UBE2M. UBE2M/Ubc12 mediates conjugation of the Ubiquitin-like protein Neural Precursor Cell Expressed, Developmentally Down-regulated 8 (NEDD8) to substrate proteins (1). Neddylation requires the coordinated activities of UBE2M/Ubc12 and the APPBPI/UBA3 heterodimer, a NEDD8-activating (E1) enzyme (2,3). The NEDD8 pathway is essential for the viability of many organisms and plays important roles in morphogenesis and cell cycle progression (4-5). UBE2M/Ubc12-dependent neddylation modulates the levels of p27/Kip1, beta-Catenin, Cdc53/Cul1, and other members of the Cullin family (6-9).

References
  1. Gong, L. et al. (1999) J. Biol. Chem. 274:12036. 
  2. Huang, D.T. et al. (2005) Mol. Cell 17:341. 
  3. Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
  4. Osaka, F. et al. (2000) EMBO J. 19:3475.
  5. Tateishi, K. et al. (2001) J. Cell Biol. 155:571. 
  6. Huang, A.M. et al. (2011) Biochem. Pharmacol. 82:35.
  7. Miller, B.W. et al. (2009) Mol. Syst. Biol. 5:315.
  8. Huang, D.T. et al. (2009) Mol. Cell 33:483.
  9. Tanaka, T. et al. (2012) Mol. Oncol. 6:267.
Entrez Gene IDs
9040 (Human); 22192 (Mouse); 361509 (Rat)
Alternate Names
EC 6.3.2; EC 6.3.2.-; hUbc12; NEDD8 carrier protein; NEDD8 protein ligase; UBC12; UBC12NEDD8-conjugating enzyme Ubc12; UbcH12; UBC-RS2; UBE2M; ubiquitin carrier protein M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12); ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog

Citations for Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing SWI/SNF
    Authors: Radko-Juettner, S;Yue, H;Myers, JA;Carter, RD;Robertson, AN;Mittal, P;Zhu, Z;Hansen, BS;Donovan, KA;Hunkeler, M;Rosikiewicz, W;Wu, Z;McReynolds, MG;Roy Burman, SS;Schmoker, AM;Mageed, N;Brown, SA;Mobley, RJ;Partridge, JF;Stewart, EA;Pruett-Miller, SM;Nabet, B;Peng, J;Gray, NS;Fischer, ES;Roberts, CWM;
    Nature
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer
    Authors: AM Cathcart, GH Bird, TE Wales, HD Herce, EP Harvey, ZJ Hauseman, CE Newman, U Adhikary, MS Prew, T Oo, S Lee, JR Engen, LD Walensky
    Nat. Chem. Biol., 2020-08-17;0(0):.
    Species: Human, N/A
    Sample Types: Peptide, Protein
    Applications: Bioassay
  3. Mdm2-mediated neddylation of pVHL blocks the induction of antiangiogenic factors
    Authors: ER Wolf, AR Mabry, B Damania, LD Mayo
    Oncogene, 2020-06-17;0(0):.
    Species: Human
    Sample Types: In Vitro
    Applications: Bioassay
  4. A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation
    Authors: H Zhou, J Lu, L Liu, D Bernard, CY Yang, E Fernandez-, K Chinnaswam, S Layton, J Stuckey, Q Yu, W Zhou, Z Pan, Y Sun, S Wang
    Nat Commun, 2017-10-27;8(1):1150.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function
    Authors: KE Coleman, M Békés, JR Chapman, SB Crist, MJ Jones, BM Ueberheide, TT Huang
    Elife, 2017-05-05;6(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. The ubiquitin-associated (UBA) domain of SCCRO/DCUN1D1 protein serves as a feedback regulator of biochemical and oncogenic activity.
    Authors: Huang G, Towe C, Choi L, Yonekawa Y, Bommelje C, Bains S, Rechler W, Hao B, Ramanathan Y, Singh B
    J Biol Chem, 2014-11-19;290(1):296-309.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  7. SCCRO3 (DCUN1D3) antagonizes the neddylation and oncogenic activity of SCCRO (DCUN1D1).
    Authors: Huang G, Stock C, Bommelje C, Weeda V, Shah K, Bains S, Buss E, Shaha M, Rechler W, Ramanathan S, Singh B
    2014-10-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates

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