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Recombinant Human NETO2 His-tag Protein, CF

Catalog #: 10975-NO Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10975-NO-050
Recombinant Human NETO2 His-tag Protein SDS-PAGE.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human NETO2 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human NETO2 His-tag, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Source
Mouse myeloma cell line, NS0-derived human NETO2 protein
Ile23-Lys345, with a C-terminal 6-His tag
Accession #
Q8NC67.1
N-terminal Sequence
Analysis
Ile23
Predicted Molecular Mass
37 kDa
SDS-PAGE
32-45 kDa, reducing conditions.

Product Datasheets

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10975-NO

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10975-NO

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE View Larger

2 μg/lane of Recombinant Human NETO2 His-tag Protein (Catalog # 10975-NO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-45 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NETO2

NETO2, or Neuropilin and tolloid-like protein 2, is a type 1 transmembrane protein belonging to the CUB (for complement C1r/C1s, Ueqf, Bmp1) domain and LDLa‑containing protein family that modulates the neuronal kainate receptors (KARs) (1-3). It is mainly expressed in neural tissues but was also reported to be expressed in several non-neural tissues and its expression has been associated with various cancers such as renal, lung, colon, cervical and colorectal cancers (4-6). Mature human NETO2 is composed of a 325 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 157 aa cytoplasmic region. Within the ECD, human NETO2 shares 98% aa identity with mouse and rat NETO2. It functions as an accessory subunit of neuronal kainate-sensitive glutamate receptors, GRIK2 and GRIK3 regulating channel activities and synaptic transmission (7).

References
  1. Fedorova, M.S. et al. (2020) Front. Genet. 11:594933. doi: 10.3389/fgene.2020.594933.
  2. Stohr, H. et al. (2002) Gene 286:223.
  3. Straub, C. et al. (2011) J. Neurosci. 31:8078.
  4. Li, Y. et al. (2019) Cancer Manag. Res. 11:5147.
  5. Niu, O. et al. (2012) Genetika. 48:506.
  6. Xu, J. et al. (2021) Int. J. Biol. Sci. 17:259.
  7. Griffith T. et al. (2015) J. Physiol. 593:4815.
Long Name
Neuropilin [NRP] And Tolloid [TLL]-like 2
Entrez Gene IDs
81831 (Human); 74513 (Mouse); 307757 (Rat)
Alternate Names
Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 2; BTCL2; FLJ10430; FLJ90456; NEOT2; NEOT2FLJ14724; NETO2; neuropilin (NRP) and tolloid (TLL)-like 2; neuropilin and tolloid-like protein 2

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