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Recombinant Human Neuroligin 1v2 (-ssB)/NLGN1v2 (-ssB), CF

Catalog #: 6446-NL Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
6446-NL-050
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Recombinant Human Neuroligin 1v2 (-ssB)/NLGN1v2 (-ssB), CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When recombinant human Neuroligin 1 is immobilized at 5.0 μg/mL, recombinant human Neurexin 1 beta Fc Chimera (Catalog # 5268-NX) binds with an apparent KD < 10 nM.
Source
Mouse myeloma cell line, NS0-derived human Neuroligin 1/NLGN1 protein
Gln46-Ser677, Deletion: aa 279-287, with a C-terminal 6-His tag
Accession #
NP_055747
N-terminal Sequence
Analysis
No results obtained: Gln46 predicted
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
70.3 kDa (monomer)
SDS-PAGE
90-100 kDa, reducing conditions

Product Datasheets

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6446-NL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6446-NL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuroligin 1/NLGN1

Neuroligin 1 (NLGN1 or NL1) is one of several type I transmembrane Neuroligin proteins that play an important role in synaptic development (1). Mature human Neuroligin 1 is an N- and O-glycosylated 120 kDa molecule that consists of a 649 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 125 aa cytoplasmic tail (2). In rat, insertion of 20 aa at splice site A and/or 9 aa at splice site B within the ECD results in NLGN1A, NLGN1B, or NLGN1AB isoforms while the NLGN1(-) isoform has neither insertion (3). This recombinant protein lacks both the splice site A and splice site B inserts and corresponds to the human equivalent of rat NLGN1(-). A protein lacking both insertions has also been predicted in chimpanzee (Accession # XP_001166442). Within the ECD, human NLGN1(-) shares 99% aa sequence identity with comparable regions of mouse and rat Neuroligin 1, respectively. Neuroligin 1 expression is restricted to postsynaptic nerve terminal membranes where it associates into dimers and tetramers (5 ‑ 7). NLGN1B and NLGN1AB are enriched at excitatory (glutamatergic) contacts, whereas NLGN1A is enriched at inhibitory (GABAergic) contacts and NLGN1(-) is expressed at both (3). The interaction of Neuroligin 1 with presynaptic membrane Neurexins promotes the maturation of presynaptic as well as postsynaptic structures (3, 4, 8 ‑ 11). Neuroligin 1 isoforms are selective in which Neurexin isoforms they bind: NLGN1B and NLGN1AB preferentially interact with Neurexin 1 beta lacking the splice site 4 insert, while NLGN1A and NLGN1(-) bind Neurexin 1 alpha and Neurexin 1 beta irrespective of the SS4 insert (12). Neurexin 1 beta (-SS4) additionally binds to LRRTM2 to trigger a second mechanism that promotes excitatory synapse development (13, 14). Neuroligin 1 mediated synaptogenesis is also enhanced by its direct binding to Thrombospondin 1 (15).

References
  1. Sudhof, T.C. (2008) Nature 455:903.
  2. Ichtchenko, K. et al. (1995) Cell 81:435.
  3. Chih, B. et al. (2006) Neuron 51:171.
  4. Dean, C. et al. (2003) Nat. Neurosci. 7:708.
  5. Comoletti, D. et al. (2006) Biochemistry 45:12816.
  6. Berninghausen, O. et al. (2007) J. Neurochem. 103:1855.
  7. Song, J.-Y. et al. (1999) Proc. Natl. Acad. Sci. 96:1100.
  8. Wittenmayer, N. et al. (2009) Proc. Natl. Acad. Sci. 106:13564.
  9. Jung, S.-Y. et al. (2010) Proc. Natl. Acad. Sci. 107:4710.
  10. Chubykin, A.A. et al. (2007) Neuron 54:919.
  11. Chih, B. et al. (2005) Science 307:1324.
  12. Boucard, A.A. et al. (2005) Neuron 48:229.
  13. Siddiqui, T.J. et al. (2010) J. Neurosci. 30:7495.
  14. Ko, J. et al. (2009) Neuron 64:791.
  15. Xu, J. et al. (2010) Nat. Neurosci. 13:22.
Entrez Gene IDs
22871 (Human); 192167 (Mouse); 116647 (Rat)
Alternate Names
EC 3.1.1; EC 3.1.1.1; KIAA1070neuroligin-1; MGC45115; Neuroligin 1; NL1; Nlg; NLGN1

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