Recombinant Human Neuroligin 4/NLGN4 Protein, CF
Recombinant Human Neuroligin 4/NLGN4 Protein, CF Summary
Product Specifications
Gln42-Ser676, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5158-NL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Neuroligin 4X/NLGN4X
Neuroligin 4 (NLGN4, NL4 or NL4X) is a 110 kDa type I transmembrane glycoprotein that is a member of the type-B carboxyesterase/lipase family of proteins (1). Neuroligins are postsynaptically expressed on neurons and initiate excitatory presynapse maturation through binding to select isoforms of beta -neurexin (1 - 3). The 816 amino acid (aa) human NLGN4 contains a 41 aa signal sequence, a 635 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 119 aa cytoplasmic tail. The ECD possesses a nonfunctional esterase homology domain through which neuroligins, except for NLGN2, interact with neurexins (4). Human NLGN4 is found on the X-chromosome. It shares 69% - 73% aa identity with NLGNs 1, 2 and 3, and 98% aa identity with NLGN4Y, a Y-chromosome-encoded neuroligin (1). Human NLGN4 ECD shares 62%, 99% and 99% aa identity with mouse, equine and canine NLGN4, respectively (5). Unlike other neuroligins, human NLGN4 does not appear to express alternate splice forms (1, 6). Crystalization of the NLGN4 ECD with and without beta -neurexin shows that NLGN4 forms a homodimer via a hydrophobic interface, but interactions with beta -neurexin are hydrophilic and calcium-dependent (4, 6). NLGNs 3 and 4 bind syntrophin-gamma 2 intracellularly (7). Mutations of NLGN4 can be associated with rare cases of autism, Asperger or Tourette syndromes (8 - 10). Mice with a loss-of-function mutation in NLGN4 show deficits in reciprocal social interactions and communication that are reminiscent of autism spectrum conditions in humans (11).
- Bollinger, M. F. et al. (2001) Biochem. J. 356:581.
- Chih, B. et al. (2005) Science 307:1324.
- Nam, C. I. and L. Chen (2005) Proc. Natl. Acad. Sci. USA 102:6137.
- Chen, X. et al. (2008) Nat. Struct. Mol. Biol. 15:50.
- Bollinger, M. F. et al. (2008) Proc. Natl. Acad. Sci. USA 105:6421.
- Fabrichny, I. P. et al. (2007) Neuron 56:979.
- Yamakawa H. et al. (2007) Biochem. Biophys. Res. Commun. 355:41.
- Jamain, S. et al. (2003) Nat. Genet. 34:27.
- Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
- Lawson-Yuen, A. et al. (2008) Eur. J. Hum. Genet. 16:614.
- Jamain, S. et al. (2008) Proc. Natl. Acad. Sci. USA 105:1710.
Citations for Recombinant Human Neuroligin 4/NLGN4 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Autism-associated variants of neuroligin 4X impair synaptogenic activity by various molecular mechanisms
Authors: T Yumoto, M Kimura, R Nagatomo, T Sato, S Utsunomiya, N Aoki, M Kitaura, K Takahashi, H Takemoto, H Watanabe, H Okano, F Yoshida, Y Nao, T Tomita
Mol Autism, 2020-09-01;11(1):68.
Species: Rat
Sample Types: In Vivo
Applications: Bioassay, In Vivo -
Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma
Authors: HS Venkatesh, LT Tam, PJ Woo, J Lennon, S Nagaraja, SM Gillespie, J Ni, DY Duveau, PJ Morris, JJ Zhao, CJ Thomas, M Monje
Nature, 2017-09-20;549(7673):533-537.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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