Recombinant Human Ninjurin-2 Protein, CF Summary
Product Specifications
Met1-Thr65, wtih a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9719-NJ
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Ninjurin-2
Ninjurin-2 (nerve injury-induced protein 2) is a 20-22 kDa member of the Ninjurin family of transmembrane (TM) proteins (1). It is expressed by multiple cell types, including Schwann cells, myenteric plexus and sensory neurons, and lymphocytes and participates in intercellular homophilic binding (1). Human Ninjurin-2 is 142 amino acids (aa) in length. It has an unusual membrane orientation. There is a 65 aa N-terminal extracellular domain (ECD) (aa 1‑65) that contains one phoshorylation site at Ser3, followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 128‑142). One potential alternate start site exists 46 aa upstream of the standard form start site. Over aa 1‑65, human Ninjurin-2 is 71% aa identical to mouse Ninjurin-2. Multiple studies have linked NINJ-2 gene polymorphism with ischemic stroke (2-4). In endothelia cells, NINJ-2 activates TLR4 signaling pathway and promote inflammation (5).
- Araki, T. and J. Milbrandt (2000) J. Neurosci. 20:187.
- Zhu, Y. et al. (2014) Thromb Thrombolysis. 38:470.
- Bis. JC. et al. (2014) PLoS One 9:e99798.
- Li, BH., et al. (2012) J Neurol Sci 316:116.
- Wang, J. et al. (2017) Cell Signal 35:231.
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