Recombinant Human NKG2C/CD159c Fc Chimera Protein, CF
Recombinant Human NKG2C/CD159c Fc Chimera Protein, CF Summary
Product Specifications
| MD | Human IgG1 (Pro100-Lys330) | IEGR | Human NKG2C (Glu98-Leu231) Accession # P26717 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
138-NK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 400 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human NKG2C/CD159c Fc Chimera (Catalog # 138-NK) binds Recombinant Human CD94 (Catalog # 9270-CD) in a functional ELISA. The ED50 for this effect is 0.3-2.4 µg/mL.
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2 μg/lane of Recombinant Human NKG2C/CD159c (Catalog # 138-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-62 kDa and 110-120 kDa, respectively.
Reconstitution Calculator
Background: NKG2C/CD159c
Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C+ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12).
- Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
- Tassi I. et al. (2006) Immnunol Rev. 214:92.
- Lanier, L.L. (2008) Nat. Immunol. 9:495.
- Schleinitz, N. et al. (2010) Immunology. 174:2878.
- Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
- Braud, V.M. et al. (1998) Nature. 391:795.
- Lanier, L.L. (1998) Immunity 8:693.
- Vance, R.E. et al. (1999) J Exp Med 190:1801.
- Kaiser B.K. et al. (2005) J Immunol 174:2878.
- Bellón T. et al. (1999) J Immunol 162:3996.
- Pupuleku A. et al. (2017) Front Immunol. 8:1317.
- Hammer Q. et al. (2018) Nat Immunol. 19:453.
Citation for Recombinant Human NKG2C/CD159c Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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A human autoimmune organoid model reveals IL-7 function in coeliac disease
Authors: Santos, AJM;van Unen, V;Lin, Z;Chirieleison, SM;Ha, N;Batish, A;Chan, JE;Cedano, J;Zhang, ET;Mu, Q;Guh-Siesel, A;Tomaske, M;Colburg, D;Varma, S;Choi, SS;Christophersen, A;Baghdasaryan, A;Yost, KE;Karlsson, K;Ha, A;Li, J;Dai, H;Sellers, ZM;Chang, HY;Dunn, JCY;Zhang, BM;Mellins, ED;Sollid, LM;Fernandez-Becker, NQ;Davis, MM;Kuo, CJ;
Nature
Species: Human
Sample Types: Organoid
Applications: Bioassay
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