Recombinant Human NKG2E Fc Chimera Protein, CF Summary
Product Specifications
| MD | Human IgG1 (Pro100-Lys330) | IEGR | Human NKG2E (Glu98-Ser240) Accession # Q07444-1 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9759-NK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human CD94 (Catalog # 9270-CD) is coated at 1 µg/mL, Recombinant Human NKG2E Fc Chimera (Catalog # 9759-NK) binds with an ED50 of 1-7 µg/mL.
Reconstitution Calculator
Background: NKG2E
Human NKG2E (NK cell Group 2 isoform E; Killer cell lectin-like receptor subfamily C, member 3) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). Human NKG2E consist of a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 147 aa extracellular domain (ECD). Within the ECD, human NKG2E shares 40% sequence identity with mouse NKG2E. NKG2E-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (7, 8). Triggering the NKG2E-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (1, 7, 9). Over-expression of KLRC3/NKG2E in glioblastomas may play a major role in glioblastoma aggressiveness and recurrence (10).
- Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
- Tassi, I. et al. (2006) Immnunol Rev. 214:92.
- Lanier, L.L. (2008) Nat. Immunol. 9:495.
- Schleinitz, N. et al. (2010) Immunology. 174:2878.
- Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
- Braud, V.M. et al. (1998) Nature. 391:795.
- Vance, R.E. et al. (1999) J Exp Med 190:1801.
- Kaiser, B.K. et al. (2005) J Immunol 174:2878.
- Bellón, T. et al. (1999) J Immunol 162:3996.
- Cheray, M. et al. (2017) J Cell Mol Med 21:244.
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