Recombinant Human Notch-4 Fc Chimera Protein, CF

Catalog #: 9909-NT Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9909-NT-050

Recombinant Human Notch-4 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Notch-4 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human DLL4 (Catalog # 1506-D4) is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Notch-4 Human Fc Chimera that produces 50% of the optimal binding response is 0.5-2.5 μg/mL.

Source

Trichoplusia ni, T. ni (baculovirus)-derived human Notch-4 protein

Human Notch‑4 (Arg24-Glu549) Accession # Q99466-1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q99466-1

N-terminal Sequence
Analysis

Arg24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

82 kDa

SDS-PAGE

94-116 kDa, reducing conditions

Product Datasheets

9909-NT

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9909-NT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human DLL4 (Catalog # 1506-D4) is immobilized at 2 ug/mL, Recombinant Human Notch-4 Fc Chimera (Catalog # 9909-NT) binds with an ED₅₀ of 0.5-2.5 µg/mL.

SDS-PAGE

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2μg/lane of Recombinant Human Notch‑4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 94-116 kDa and 170-200 kDa, respectively.

Reconstitution Calculator

Background: Notch-4

Human Notch-4 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes (1-3). Human Notch-4 is synthesized as a 2003 amino acid (aa) precursor that contains a 23 aa signal sequence, a 1424 aa extracellular domain (ECD) with 29 EGF-like repeats and three Lin-12/Notch repeats (LNR), a 21 aa transmembrane (TM) segment and a 535 aa cytoplasmic domain containing five ankyrin repeats (1, 3). Within the expressed region of this product (including EGF domains 1-13) human Notch-4 shares 84%, 83%, 51%, and 48% aa sequence identity with mouse Notch-4, rat Notch-4, human Notch-2 and human Notch-1 respectively. The 11th and 12th EGF-like repeats bind ligands including Jagged and Delta-like families in humans (4). Delta-like ligand-4 (DLL4)-Notch-4 signaling correlates to angiogenesis in glioblastomas (5). Notch-4 has been identified as a key player in invasive cancers such as breast cancer, hepatocellular carcinoma, melanoma, and prostate cancer (6-9). Notch-4 expression in 3T3L preadipocytes enhanced their proliferation and adipogenesis though up‑regulation of adipogenic genes such as C/EBP alpha, PPAR gamma, aP2, LPL and HSL (10). It has also been shown to be involved in proliferation of gastric cancer though Wnt-1/ beta -catenin signaling regulation (11).

References