Recombinant Human NT-3 Protein, CF

Name: Recombinant Human NT-3 Protein, CF
Brand: R&D Systems
SKU: 11346-N3
Price: 273 USD

Catalog #: 11346-N3 Datasheet / COA / SDS

Newer Version Available:

NEW

(HEK293 expressed), Analyzed by SEC-MALS. 11563-N3, a new product from mammalian cell line is available

Catalog #	Availability	Size / Price	Qty
11346-N3-010
11346-N3-050
11346-N3-100
11346-N3-01M

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Recombinant Human NT‑3 Protein SEC-MALS.

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All NT-3 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human NT-3 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED₅₀ for this effect is 5.00-60.0 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human NT-3 protein
Tyr139-Thr257

Accession #

P20783.1

N-terminal Sequence
Analysis

Tyr139

Predicted Molecular Mass

14 kDa

SDS-PAGE

12-15 kDa, under reducing conditions.

Product Datasheets

11346-N3

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11346-N3

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS

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Recombinant Human NT-3 Protein (Catalog # 11346-N3) has a molecular weight (MW) of 28.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Bioactivity

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Recombinant Human NT‑3 (Catalog # 11346-N3) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED₅₀ for this effect is 5.00-60.0 ng/mL.

Reconstitution Calculator

Background: NT-3

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

References