Home / Oncostatin M/OSM / Recombinant Human Oncostatin M (OSM) Protein

Recombinant Human Oncostatin M (OSM) Protein

Catalog #: 295-OM
77 Citations 3 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
295-OM-010/CF
295-OM-050/CF
295-OM-01M/CF

With Carrier

Catalog # Availability Size / Price Qty
295-OM-010
295-OM-050
295-OM-01M
295-OM-250
Graph showing dose-dependent bioactivity of human Oncostatin M protein
1 Image
Product Details
Citations (77)
FAQs
Reviews (3)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Oncostatin M (OSM) Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.3 ng/mL.
Source
E. coli-derived human Oncostatin M/OSM protein
Ala26-Arg221
Accession #
P13725
Predicted Molecular Mass
22 kDa

Product Datasheets

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295-OM (with carrier)

Product Datasheet
COA
SDS

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295-OM/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

295-OM

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

295-OM/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Graph showing dose-dependent bioactivity of human Oncostatin M protein View Larger

Recombinant human Oncostatin M / OSM (295-OM) induces proliferation in the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.05-0.3 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Oncostatin M/OSM

OSM is a cytokine originally isolated from medium conditioned by PMA-treated U-937 human histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The human OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and pro-OSM are equally active in radio-receptor assays, the mature OSM is 5- to 60-fold more active in growth inhibition assays. Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM.

OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. The gp130, a signal transducing component ( beta  subunit) of the IL-6, LIF and CNTF receptor complexes, was identified as a low-affinity OSM receptor that does not transduce OSM signals. The low affinity LIF receptor (LIF R, a gp130-related protein) has now been identified to be a component of a high-affinity OSM receptor that will transduce OSM signals. Since OSM is also active on cells that do not express LIF R, a specific OSM receptor that does not involve LIF R must also exist. Besides its growth inhibitory activities on human A375 melanoma and mouse M1 myeloid leukemic cells, as well as on other solid tumor cells, OSM also has growth stimulatory activities on normal fibroblasts, AIDS-Kaposi’s sarcoma cells, and a human erythroleukemia cell line, TF-1. Other OSM-mediated activities reported to date include: stimulation of plasminogen activator activity in cultured bovine aortic endothelial cells; regulation of IL-6 expression in human endothelial cells; and stimulation of LDL uptake and up-regulation of cell surface LDL receptors in HepG2 cells.

Entrez Gene IDs
5008 (Human); 18413 (Mouse)
Alternate Names
MGC20461; oncostatin M; oncostatin-M; OSM

Citations for Recombinant Human Oncostatin M (OSM) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

77 Citations: Showing 1 - 10
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  1. Engineered Interleukin-6-derived cytokines recruit artificial receptor complexes and disclose CNTF signaling via the OSMR
    Authors: Rafii, P;Cruz, PR;Ettich, J;Seibel, C;Padrini, G;Wittich, C;Lang, A;Petzsch, P;Köhrer, K;Moll, JM;Floss, DM;Scheller, J;
    The Journal of biological chemistry
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Improvements in Maturity and Stability of 3D iPSC-Derived Hepatocyte-like Cell Cultures
    Authors: Suominen, S;Hyypijev, T;Venäläinen, M;Yrjänäinen, A;Vuorenpää, H;Lehti-Polojärvi, M;Räsänen, M;Seppänen, A;Hyttinen, J;Miettinen, S;Aalto-Setälä, K;Viiri, LE;
    Cells
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. A defined roadmap of radial glia and astrocyte differentiation from human pluripotent stem cells
    Authors: Jovanovic, VM;Weber, C;Slamecka, J;Ryu, S;Chu, PH;Sen, C;Inman, J;De Sousa, JF;Barnaeva, E;Hirst, M;Galbraith, D;Ormanoglu, P;Jethmalani, Y;Mercado, JC;Michael, S;Ward, ME;Simeonov, A;Voss, TC;Tristan, CA;Singeç, I;
    Stem cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Reversal of liver failure using a bioartificial liver device implanted with clinical-grade human-induced hepatocytes
    Authors: Y Wang, Q Zheng, Z Sun, C Wang, J Cen, X Zhang, Y Jin, B Wu, T Yan, Z Wang, Q Gu, X Lv, J Nan, Z Wu, W Sun, G Pan, L Zhang, L Hui, X Cai
    Cell Stem Cell, 2023-04-13;0(0):.
    Species: Human
    Sample Types:
  5. Coronary artery disease patient-derived iPSC-hepatocytes have distinct miRNA profile that may alter lipid metabolism
    Authors: A Alexanova, E Raitoharju, J Valtonen, K Aalto-Setä, LE Viiri
    Scientific Reports, 2023-01-30;13(1):1706.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation, Differentiation
  6. The HASTER lncRNA promoter is a cis-acting transcriptional stabilizer of HNF1A
    Authors: A Beucher, I Miguel-Esc, D Balboa, MG De Vas, MA Maestro, J Garcia-Hur, A Bernal, R Gonzalez-F, P Vargiu, H Heyn, P Ravassard, S Ortega, J Ferrer
    Nature Cell Biology, 2022-10-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. High temporal resolution proteome and phosphoproteome profiling of stem cell-derived hepatocyte development
    Authors: J Krumm, K Sekine, P Samaras, A Brazovskaj, M Breunig, R Yasui, A Kleger, H Taniguchi, M Wilhelm, B Treutlein, JG Camp, B Kuster
    Cell Reports, 2022-03-29;38(13):110604.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Formative pluripotent stem cells show features of epiblast cells poised for gastrulation
    Authors: X Wang, Y Xiang, Y Yu, R Wang, Y Zhang, Q Xu, H Sun, ZA Zhao, X Jiang, X Wang, X Lu, D Qin, Y Quan, J Zhang, N Shyh-Chang, H Wang, N Jing, W Xie, L Li
    Cell Research, 2021-02-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Pathogenic LMNA variants disrupt cardiac lamina-chromatin interactions and de-repress alternative fate genes
    Authors: PP Shah, W Lv, JH Rhoades, A Poleshko, D Abbey, MA Caporizzo, R Linares-Sa, JG Heffler, N Sayed, D Thomas, Q Wang, LJ Stanton, K Bedi, MP Morley, TP Cappola, AT Owens, KB Margulies, DB Frank, JC Wu, DJ Rader, W Yang, BL Prosser, K Musunuru, R Jain
    Cell Stem Cell, 2021-02-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs
    Authors: F Michielin, GG Giobbe, C Luni, Q Hu, I Maroni, MR Orford, A Manfredi, L Di Filippo, AL David, D Cacchiarel, P De Coppi, S Eaton, N Elvassore
    Cell Rep, 2020-12-01;33(9):108453.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study
    Authors: X Gao, R Li, P Cahan, Y Zhao, JJ Yourick, RL Sprando
    Stem Cell Res Ther, 2020-09-11;11(1):393.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  12. Plasmodium vivax liver stage assay platforms using Indian clinical isolates
    Authors: PA Subramani, N Vartak-Sha, S Sreekumar, P Mathur, B Nayer, S Dakhore, SK Basavanna, DM Kalappa, RV Krishnamur, B Mukhi, P Mishra, N Yoshida, SK Ghosh, R Shandil, S Narayanan, B Campo, K Hasegawa, AR Anvikar, N Valecha, V Sundaramur
    Malar. J., 2020-06-22;19(1):214.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
    Authors: L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen
    Cell Stem Cell, 2020-06-19;27(1):125-136.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
    Authors: NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman
    Nat. Biotechnol., 2020-02-24;0(0):.
    Species: Human
    Sample Types: Cells
    Applications: cells
  15. Deducing signaling pathways from parallel actions of arsenite and antimonite in human epidermal keratinocytes
    Authors: MA Phillips, A Cánovas, MA Rea, A Islas-Trej, JF Medrano, B Durbin-Joh, DM Rocke, RH Rice
    Sci Rep, 2020-02-19;10(1):2890.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells
    Authors: Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban
    Nat. Immunol., 2020-02-17;21(3):274-286.
    Species: Human
    Sample Types: Cells
    Applications: Cell Culture
  17. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
    Authors: L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen
    Cell Stem Cell, 2020;27(1):125-136.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. miRNA551b-3p Activates an Oncostatin Signaling Module for the Progression of Triple-Negative Breast Cancer
    Authors: D Parashar, A Geethadevi, MR Aure, J Mishra, J George, C Chen, MK Mishra, A Tahiri, W Zhao, B Nair, Y Lu, LS Mangala, C Rodriguez-, G Lopez-Bere, AKS Camara, M Liang, JS Rader, R Ramchandra, M You, AK Sood, VN Kristensen, GB Mills, S Pradeep, P Chaluvally
    Cell Rep, 2019-12-24;29(13):4389-4406.e10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  19. Adenosine Attenuates Aortic Smooth Muscle Cell Calcification through A3 Adenosine Receptor
    Authors: K Fujimoto, A Shioi, Y Miki, Y Kakutani, T Morioka, T Shoji, M Emoto, M Inaba
    Tohoku J. Exp. Med., 2019-12-01;249(4):275-283.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. In vitro metabolic zonation through oxygen gradient on a chip
    Authors: F Tonon, GG Giobbe, A Zambon, C Luni, O Gagliano, A Floreani, G Grassi, N Elvassore
    Sci Rep, 2019-09-19;9(1):13557.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Skeletal Stem Cell-Schwann Cell Circuitry in Mandibular Repair
    Authors: RE Jones, A Salhotra, KS Robertson, RC Ransom, DS Foster, HN Shah, N Quarto, DC Wan, MT Longaker
    Cell Rep, 2019-09-10;28(11):2757-2766.e5.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  22. Single cell analysis of human foetal liver captures the transcriptional profile of hepatobiliary hybrid progenitors
    Authors: JM Segal, D Kent, DJ Wesche, SS Ng, M Serra, B Oulès, G Kar, G Emerton, SJI Blackford, S Darmanis, R Miquel, TV Luong, R Yamamoto, A Bonham, W Jassem, N Heaton, A Vigilante, A King, R Sancho, S Teichmann, SR Quake, H Nakauchi, ST Rashid
    Nat Commun, 2019-07-26;10(1):3350.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  23. Function and Immunogenicity of Gene-corrected iPSC-derived Hepatocyte-Like Cells in Restoring Low Density Lipoprotein Uptake in Homozygous Familial Hypercholesterolemia
    Authors: H Okada, C Nakanishi, S Yoshida, M Shimojima, J Yokawa, M Mori, H Tada, T Yoshimuta, K Hayashi, T Yamano, R Hanayama, M Yamagishi, MA Kawashiri
    Sci Rep, 2019-03-18;9(1):4695.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. An integrated chromatin accessibility and transcriptome landscape of human pre-implantation embryos
    Authors: L Liu, L Leng, C Liu, C Lu, Y Yuan, L Wu, F Gong, S Zhang, X Wei, M Wang, L Zhao, L Hu, J Wang, H Yang, S Zhu, F Chen, G Lu, Z Shang, G Lin
    Nat Commun, 2019-01-21;10(1):364.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Recapitulation of methotrexate hepatotoxicity with induced pluripotent stem cell-derived hepatocytes from patients with rheumatoid arthritis
    Authors: J Kim, Y Kim, J Choi, H Jung, K Lee, J Kang, N Park, YA Rim, Y Nam, JH Ju
    Stem Cell Res Ther, 2018-12-29;9(1):357.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Metformin reveals a mitochondrial copper addiction of mesenchymal cancer cells
    Authors: S Müller, A Versini, F Sindikubwa, G Belthier, S Niyomchon, J Pannequin, L Grimaud, T Cañeque, R Rodriguez
    PLoS ONE, 2018-11-06;13(11):e0206764.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Inflammatory cytokine oncostatin M induces endothelial activation in macro- and microvascular endothelial cells and in APOE*3Leiden.CETP mice
    Authors: D van Keulen, MG Pouwer, G Pasterkamp, AJ van Gool, MD Sollewijn, HMG Princen, D Tempel
    PLoS ONE, 2018-10-01;13(10):e0204911.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes
    Authors: A Skrzypczyk, S Kehr, I Krystel, SH Bernhart, S Giri, A Bader, PF Stadler
    Stem Cells Int, 2018-08-19;2018(0):5692840.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Optimal Hypoxia Regulates Human iPSC-Derived Liver Bud Differentiation through Intercellular TGFB Signaling
    Authors: H Ayabe, T Anada, T Kamoya, T Sato, M Kimura, E Yoshizawa, S Kikuchi, Y Ueno, K Sekine, JG Camp, B Treutlein, A Ferguson, O Suzuki, T Takebe, H Taniguchi
    Stem Cell Reports, 2018-07-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Successful reprogramming of cellular protein production through mRNA delivered by functionalized lipid nanoparticles
    Authors: M Yanez Arte, T Kjellman, S Bartesaghi, S Wallin, X Wu, AJ Kvist, A Dabkowska, N Székely, A Radulescu, J Bergenholt, L Lindfors
    Proc. Natl. Acad. Sci. U.S.A., 2018-03-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications
    Authors: J Ou, JM Ball, Y Luan, T Zhao, KJ Miyagishim, Y Xu, H Zhou, J Chen, DK Merriman, Z Xie, BS Mallon, W Li
    Cell, 2018-03-22;0(0):.
    Species: Squirrel
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Magnetic Targeting of Stem Cell Derivatives Enhances Hepatic Engraftment into Structurally Normal Liver
    Authors: WS Fagg, N Liu, MJ Yang, K Cheng, E Chung, JS Kim, G Wu, J Fair
    Cell Transplant, 2017-12-01;26(12):1868-1877.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Stem Cells from Cryopreserved Human Dental Pulp Tissues Sequentially Differentiate into Definitive Endoderm and Hepatocyte-Like Cells in vitro
    Authors: YJ Han, YH Kang, SB Shivakumar, D Bharti, YB Son, YH Choi, WU Park, JH Byun, GJ Rho, BW Park
    Int J Med Sci, 2017-11-02;14(13):1418-1429.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Stat3 as a potential therapeutic target for rheumatoid arthritis
    Authors: T Oike, Y Sato, T Kobayashi, K Miyamoto, S Nakamura, Y Kaneko, S Kobayashi, K Harato, H Saya, M Matsumoto, M Nakamura, Y Niki, T Miyamoto
    Sci Rep, 2017-09-08;7(1):10965.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Multilineage communication regulates human liver bud development from pluripotency
    Authors: JG Camp, K Sekine, T Gerber, H Loeffler-W, H Binder, M Gac, S Kanton, J Kageyama, G Damm, D Seehofer, L Belicova, M Bickle, R Barsacchi, R Okuda, E Yoshizawa, M Kimura, H Ayabe, H Taniguchi, T Takebe, B Treutlein
    Nature, 2017-06-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes
    Authors: Q Li, AP Hutchins, Y Chen, S Li, Y Shan, B Liao, D Zheng, X Shi, Y Li, WY Chan, G Pan, S Wei, X Shu, D Pei
    Nat Commun, 2017-05-03;8(0):15166.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Large, Diverse Population Cohorts of hiPSCs and Derived Hepatocyte-like Cells Reveal Functional Genetic Variation at Blood Lipid-Associated Loci
    Authors: EE Pashos, Y Park, X Wang, A Raghavan, W Yang, D Abbey, DT Peters, J Arbelaez, M Hernandez, N Kuperwasse, W Li, Z Lian, Y Liu, W Lv, SL Lytle-Gabb, DH Marchadier, P Rogov, J Shi, KJ Slovik, IM Stylianou, L Wang, R Yan, X Zhang, S Kathiresan, SA Duncan, TS Mikkelsen, EE Morrisey, DJ Rader, CD Brown, K Musunuru
    Cell Stem Cell, 2017-04-06;20(4):558-570.e10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Mammalian target of rapamycin inhibitors, temsirolimus and torin 1, attenuate stemness-associated properties and expression of mesenchymal markers promoted by phorbol-myristate-acetate and oncostatin-M in glioblastoma cells
    Authors: G Chandrika, K Natesh, D Ranade, A Chugh, P Shastry
    Tumour Biol., 2017-03-01;39(3):1010428317695.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Patient-specific hepatocyte-like cells derived from induced pluripotent stem cells model pazopanib-mediated hepatotoxicity
    Authors: Y Choudhury, YC Toh, J Xing, Y Qu, J Poh, L Huan, HS Tan, R Kanesvaran, H Yu, MH Tan
    Sci Rep, 2017-01-25;7(0):41238.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Porcine Reproductive and Respiratory Syndrome Virus Antagonizes JAK/STAT3 Signaling via Nsp5 by Inducing STAT3 Degradation
    Authors: Yan-Jin Zhang
    J. Virol., 2017-01-18;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Human Stem Cell-Derived Endothelial-Hepatic Platform for Efficacy Testing of Vascular-Protective Metabolites from Nutraceuticals
    Authors: BC Narmada, YT Goh, H Li, S Sinha, H Yu, C Cheung
    Stem Cells Transl Med, 2016-10-07;6(3):851-863.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFN?-Primed Equine Mesenchymal Stem Cells
    Authors: Danièle Noel
    Front Immunol, 2016-09-27;7(0):392.
    Species: Equine
    Sample Types: Cartilage Explants
    Applications: Bioassay
  43. Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow
    Nat Cell Biol, 2016-09-19;18(10):1078-1089.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Murine Oncostatin M acts via Leukemia Inhibitory Factor Receptor to phosphorylate STAT3 but not STAT1, an effect that protects bone mass
    J Biol Chem, 2016-08-18;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Human induced pluripotent stem cell-derived hepatic cell lines as a new model for host interaction with hepatitis B virus
    Sci Rep, 2016-07-08;6(0):29358.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  46. Generation of iPSCs as a Pooled Culture Using Magnetic Activated Cell Sorting of Newly Reprogrammed Cells.
    Authors: Yang W, Liu Y, Slovik K, Wu J, Duncan S, Rader D, Morrisey E
    PLoS ONE, 2015-08-17;10(8):e0134995.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. Modulators of hepatic lipoprotein metabolism identified in a search for small-molecule inducers of tribbles pseudokinase 1 expression.
    Authors: Nagiec M, Skepner A, Negri J, Eichhorn M, Kuperwasser N, Comer E, Muncipinto G, Subramanian A, Clish C, Musunuru K, Duvall J, Foley M, Perez J, Palmer M
    PLoS ONE, 2015-03-26;10(3):e0120295.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  48. RelB/p50 complexes regulate cytokine-induced YKL-40 expression.
    Authors: Bhardwaj R, Yester J, Singh S, Biswas D, Surace M, Waters M, Hauser K, Yao Z, Boyce B, Kordula T
    J Immunol, 2015-02-13;194(6):2862-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Regulation of IL-17A responses in human airway smooth muscle cells by Oncostatin M.
    Authors: Kwofie K, Scott M, Rodrigues R, Guerette J, Radford K, Nair P, Richards C
    Respir Res, 2015-02-07;16(0):14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. The expression and regulation of chemerin in the epidermis.
    Authors: Banas M, Zegar A, Kwitniewski M, Zabieglo K, Marczynska J, Kapinska-Mrowiecka M, LaJevic M, Zabel B, Cichy J
    PLoS ONE, 2015-02-06;10(2):e0117830.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  51. DMSO efficiently down regulates pluripotency genes in human embryonic stem cells during definitive endoderm derivation and increases the proficiency of hepatic differentiation.
    Authors: Czysz, Katherin, Minger, Stephen, Thomas, Nick
    PLoS ONE, 2015-02-06;10(2):e0117689.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  52. Oncostatin M regulates SOCS3 mRNA stability via the MEK-ERK1/2-pathway independent of p38(MAPK)/MK2.
    Authors: Ehlting C, Bohmer O, Hahnel M, Thomas M, Zanger U, Gaestel M, Knoefel W, Schulte Am Esch J, Haussinger D, Bode J
    Cell Signal, 2015-01-03;27(3):555-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. Ethanol negatively regulates hepatic differentiation of hESC by inhibition of the MAPK/ERK signaling pathway in vitro.
    Authors: Gao W, Zhou P, Ma X, Tschudy-Seney B, Chen J, Magner N, Revzin A, Nolta J, Zern M, Duan Y
    PLoS ONE, 2014-11-13;9(11):e112698.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  54. Cancer-associated adipose tissue promotes breast cancer progression by paracrine oncostatin M and Jak/STAT3 signaling.
    Authors: Lapeire L, Hendrix A, Lambein K, Van Bockstal M, Braems G, Van Den Broecke R, Limame R, Mestdagh P, Vandesompele J, Vanhove C, Maynard D, Lehuede C, Muller C, Valet P, Gespach C, Bracke M, Cocquyt V, Denys H, De Wever O
    Cancer Res, 2014-09-24;74(23):6806-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  55. The role of bronchial epithelial cells in the pathogenesis of COPD in Z-alpha-1 antitrypsin deficiency.
    Authors: Pini L, Tiberio L, Venkatesan N, Bezzi M, Corda L, Luisetti M, Ferrarotti I, Malerba M, Lomas D, Janciauskiene S, Vizzardi E, Modina D, Schiaffonati L, Tantucci C
    Respir Res, 2014-09-14;15(0):112.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  56. Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M.
    Authors: Rabeony H, Petit-Paris I, Garnier J, Barrault C, Pedretti N, Guilloteau K, Jegou J, Guillet G, Huguier V, Lecron J, Bernard F, Morel F
    PLoS ONE, 2014-07-10;9(7):e101937.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  57. Oncostatin M acting via OSMR, augments the actions of IL-1 and TNF in synovial fibroblasts.
    Authors: Le Goff B, Singbrant S, Tonkin B, Martin T, Romas E, Sims N, Walsh N
    Cytokine, 2014-04-22;68(2):101-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  58. Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant.
    Authors: Takebe, Takanori, Zhang, Ran-Ran, Koike, Hiroyuki, Kimura, Masaki, Yoshizawa, Emi, Enomura, Masahiro, Koike, Naoto, Sekine, Keisuke, Taniguchi, Hideki
    Nat Protoc, 2014-01-23;9(2):396-409.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  59. CCAAT/enhancer binding protein-mediated regulation of TGFbeta receptor 2 expression determines the hepatoblast fate decision.
    Authors: Takayama K, Kawabata K, Nagamoto Y, Inamura M, Ohashi K, Okuno H, Yamaguchi T, Tashiro K, Sakurai F, Hayakawa T, Okano T, Furue M, Mizuguchi H
    Development, 2013-11-27;141(1):91-100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Increased ERK signalling promotes inflammatory signalling in primary airway epithelial cells expressing Z alpha1-antitrypsin.
    Authors: van 't Wout E, Dickens J, van Schadewijk A, Haq I, Kwok H, Ordonez A, Murphy G, Stolk J, Lomas D, Hiemstra P, Marciniak S
    Hum Mol Genet, 2013-10-04;23(4):929-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  61. Long-term self-renewal of human ES/iPS-derived hepatoblast-like cells on human laminin 111-coated dishes.
    Authors: Takayama K, Nagamoto Y, Mimura N, Tashiro K, Sakurai F, Tachibana M, Hayakawa T, Kawabata K, Mizuguchi H
    Stem Cell Reports, 2013-10-03;1(4):322-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  62. Production of hepatocyte-like cells from human pluripotent stem cells.
    Authors: Hannan, Nicholas, Segeritz, Charis-P, Touboul, Thomas, Vallier, Ludovic
    Nat Protoc, 2013-01-01;8(2):430-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  63. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  64. Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells.
    Authors: Roelandt P, Pauwelyn KA, Sancho-Bru P
    PLoS ONE, 2010-08-11;5(8):e12101.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  65. Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer.
    Authors: Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM
    Mol. Cell. Biol., 2010-06-28;17(0):4175.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  66. Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis.
    Authors: Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F
    J. Immunol., 2010-03-24;184(0):5263-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  67. Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
    Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
    Int. J. Cancer, 2009-07-15;125(2):367-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  68. Cytokine-induced CEACAM1 expression on keratinocytes is characteristic for psoriatic skin and contributes to a prolonged lifespan of neutrophils.
    Authors: Rahmoun M, Moles JP, Pedretti N, Mathieu M, Fremaux I, Raison-Peyron N, Lecron JC, Yssel H, Pene J
    J. Invest. Dermatol., 2008-10-09;129(3):671-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  69. Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps.
    Authors: Kato A, Peters A, Suh L, Carter R, Harris KE, Chandra R, Conley D, Grammer LC, Kern R, Schleimer RP
    J. Allergy Clin. Immunol., 2008-04-14;121(6):1385-92, 1392.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  70. Bradykinin potentiates cytokine-induced prostaglandin biosynthesis in osteoblasts by enhanced expression of cyclooxygenase 2, resulting in increased RANKL expression.
    Authors: Brechter AB, Lerner UH
    Arthritis Rheum., 2007-03-01;56(3):910-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  71. Oncostatin M enhances CCL21 expression by microvascular endothelial cells and increases the efficiency of dendritic cell trafficking to lymph nodes.
    Authors: Sugaya M, Fang L, Cardones AR, Kakinuma T, Jaber SH, Blauvelt A, Hwang ST
    J. Immunol., 2006-12-01;177(11):7665-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  72. Oncostatin M induces angiogenesis and cartilage degradation in rheumatoid arthritis synovial tissue and human cartilage cocultures.
    Authors: Fearon U, Mullan R, Markham T, Connolly M, Sullivan S, Poole AR, FitzGerald O, Bresnihan B, Veale DJ
    Arthritis Rheum., 2006-10-01;54(10):3152-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  73. Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro.
    Authors: Baharvand H, Hashemi SM, Kazemi Ashtiani S, Farrokhi A
    Int. J. Dev. Biol., 2006-01-01;50(7):645-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  74. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
    Authors: He W, Gong K, Smith DK, Ip NY
    FEBS Lett., 2005-08-15;579(20):4317-23.
    Species: Human
    Sample Types: Peptide, Whole Cells
    Applications: Binding Assay, Bioassay
  75. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002-11-01;169(9):5244-51.
    Species: Human
    Sample Types: N/A, Peritoneal Fluid
    Applications: Bioassay, ELISA (Standard)
  76. Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts.
    Authors: Scaffidi AK, 2019, Mutsaers SE, Moodley YP, McAnulty RJ, Laurent GJ, Thompson PJ, Knight DA
    3821-3828, 2002-07-01;136(5):793-801.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  77. Coexpression of oncostatin M and its receptors and evidence for STAT3 activation in human ovarian carcinomas.
    Authors: Savarese TM, Campbell CL, McQuain C, Mitchell K, Guardiani R, Quesenberry PJ, Nelson BE
    Cytokine, 2002-03-21;17(6):324-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What are the differences between Recombinant Human Oncostatin M Protein (Catalog # 295-OM) and Recombinant Human Oncostatin M (HEK293-expressed) Protein (Catalog # 8475-OM)?

    • Recombinant Human Oncostatin M Protein (Catalog # 295-OM) and Recombinant Human Oncostatin M (HEK293-expressed) Protein (Catalog # 8475-OM) share the same protein sequence, Ala26-Arg221 of Accession # P13725, but Catalog # 295-OM was expressed in E. coli and Catalog # 8475-OM was expressed in HEK293 cells. They are offered in formulations with or without BSA as a carrier protein.

      Both proteins are routinely tested and have the same activity range in our established QC bioassay: "Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells." The ED50 for this effect is 0.05-0.3 ng/mL. Catalog # 8475-OM is produced in a mammalian expression system so we would expect it to be glycosylated. Catalog # 295-OM is produced in E coli and is not glycosylated.

      If endotoxin levels are of concern for your application, we would recommend selecting the Catalog # 295-OM as it has an endotoxin threshold of <0.01 EU per 1 μg of protein. Catalog # 8475-OM has a higher endotoxin threshold of <0.10 EU per 1 μg of the protein.  That said, if it is important to use a mammalian expressed protein like Catalog # 8475-OM in your assay system, and you require endortoxin lower than <0.10 EU/1 μg of the protein please consult technical service to see if custom testing is an option. We also recommend reviewing the references under the Citations tab on the respective product pages to check the types of applications in which these two proteins have been evaluated.

  2. Is Recombinant Human Oncostatin M Protein (Catalog # 295-OM) considered the the pre-OSM peptide or mature protein?

    • Recombinant Human Oncostatin M Protein (Catalog # 295-OM) is comprised of amino acids Ala26-Arg221 of accession # P13725.  This region is the 196 residue mature form of OSM. 

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Recombinant Human Oncostatin M (OSM) Protein
By Anonymous on 05/20/2022
Application: Stem/Immune cell maintenance or differentiation
Recombinant Human Oncostatin M (OSM) Protein
By Danny Tam on 08/20/2018
Application: Media additive for protein or antibody production
Recombinant Human Oncostatin M (OSM) Protein
By Danny Tam on 12/21/2017
Application: In vitro bioactivity in cell culture