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Recombinant Human PD-ECGF/Thymidine Phosphorylase Protein

Catalog #: 229-PE
2 Citations Datasheet / COA / SDS

Discontinued Product

229-PE has been discontinued.
View all PD-ECGF/Thymidine Phosphorylase products.
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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human PD-ECGF/Thymidine Phosphorylase Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human PD-ECGF/Thymidine Phosphorylase protein
Ala11-Gln482
Accession #
P19971
N-terminal Sequence
Analysis
Ala11
Predicted Molecular Mass
49 kDa

Product Datasheets

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229-PE (with carrier)

Product Datasheet
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229-PE/CF (carrier free)

Discontinued Product

229-PE

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Background: PD-ECGF/Thymidine Phosphorylase

PD-ECGF, also known as TPase and gliostatin, is produced by placenta, platelets, liver, lung, spleen, lymph nodes, and peripheral lymphocytes. It is overexpressed by many tumors in response to chemical or physical stress. PD-ECGF promotes endothelial cell proliferation and chemotaxis and promotes angiogenesis. PD-ECGF is a key enzyme in the pyrimidine nucleoside salvage pathway. It also metabolizes and inactivates chemotherapeutic drugs such as 5-fluorouracil and its derivatives. The human PD-ECGF cDNA encodes a 482 amino acid (aa) polypeptide with a 10 aa propeptide. The protein is localized mostly within the producer cells. N-terminal truncation, resulting in proteins lacking 10 aa and 6 aa, has been observed in PD-ECGF purified from platelets and placenta, respectively. In solution, PD-ECGF exists as a non-disulfide linked homodimer. The latter protein was purified from quiescent astrocytes and described as an astrocyte and astrocytoma cell growth inhibitor based on its ability to inhibit 3H-thymidine incorporation.

References
  1. Haraguchi, M. et al. (1994) Nature 368:198.
  2. Toi, M. et al. (2005) Lancet Oncol. 6:158.
  3. Akiyama, S. et al. (2004) Canc. Sci. 95:851.
Long Name
Platelet-derived Endothelial Cell Growth Factor
Entrez Gene IDs
1890 (Human); 72962 (Mouse)
Alternate Names
dThdPase; EC 2.4.2; EC 2.4.2.4; ECGF; ECGF1; ECGF1TP; endothelial cell growth factor 1 (platelet-derived); Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine Phosphorylase; TPase; TYMP

Citations for Recombinant Human PD-ECGF/Thymidine Phosphorylase Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Glucocorticoids Suppress Mitochondrial Oxidant Production via Upregulation of Uncoupling Protein 2 in Hyperglycemic Endothelial Cells
    PLoS ONE, 2016-04-29;11(4):e0154813.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Regulation of vascular endothelial growth factor expression in human colon cancer by insulin-like growth factor-I.
    Authors: Akagi Y, Liu W, Zebrowski B, Xie K, Ellis LM
    Cancer Res., 1998-09-01;58(17):4008-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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