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Recombinant Human PDGF-AB Protein, CF

Catalog #: 222-AB
12 Citations 1 Review Datasheet / COA / SDS
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222-AB-010
222-AB-050
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Citations (12)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human PDGF-AB Protein, CF Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 15-75 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002).
Source
E. coli-derived human PDGF-AB protein
Human PDGF-A
(Ser87 - Thr211)
Accession # P04085
Human PDGF-B
(Ser82 - Thr190)
Accession # P01127
N-terminus C-terminus
Accession #
P04085.1 (A chain) & P01127.1 (B chain)
N-terminal Sequence
Analysis
Ser87 (A chain) & Ser82 (B chain)
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
14.3 kDa (A chain) & 12.3 kDa (B chain)
SDS-PAGE
17.5 kDa (A chain) & 12.5 kDa (B chain), reducing conditions

Product Datasheets

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222-AB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

222-AB

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF-AB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Long Name
Platelet-derived Growth Factor AB
Alternate Names
PDGFAB; PDGF-AB

Citations for Recombinant Human PDGF-AB Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. The Fibrotic Phenotype of Human Precision-Cut Lung Slices Is Maintained after Cryopreservation
    Authors: Marimoutou, M;Patel, V;Kim, JH;Schaible, N;Alvarez, J;Hughes, J;Obermok, M;Rodríguez, CI;Kallarakal, T;Suki, B;Amin, K;Krishnan, R;Behrsing, HP;
    Toxics
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Fibroblasts are not just fibroblasts: clear differences between dermal and pulmonary fibroblasts' response to fibrotic growth factors
    Authors: Madsen, SF;Sand, JMB;Juhl, P;Karsdal, M;Thudium, CS;Siebuhr, AS;Bay-Jensen, AC;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Long intergenic non-coding RNAs regulate human lung fibroblast function: Implications for idiopathic pulmonary fibrosis
    Authors: MR Hadjichara, BT Roux, E Csomor, CA Feghali-Bo, LA Murray, DL Clarke, MA Lindsay
    Sci Rep, 2019-04-15;9(1):6020.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics
    Authors: SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede
    Sci Rep, 2017-11-27;7(1):16439.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  5. Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3
    Authors: H He, AE Kuriyan, CW Su, M Mahabole, Y Zhang, YT Zhu, HW Flynn, JM Parel, SC Tseng
    Sci Rep, 2017-03-02;7(0):43736.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Activation of platelet-derived growth factor receptor alpha contributes to liver fibrosis.
    Authors: Hayes B, Riehle K, Shimizu-Albergine M, Bauer R, Hudkins K, Johansson F, Yeh M, Mahoney W, Yeung R, Campbell J
    PLoS ONE, 2014-03-25;9(3):e92925.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch.
    Authors: Ding W, Knox TR, Tschumper RC
    Blood, 2010-07-06;116(16):2984-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells.
    Authors: Ball SG, Bayley C, Shuttleworth CA, Kielty CM
    Biochem. J., 2010-03-15;427(1):29-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
    Authors: Li R, Maminishkis A, Wang FE, Miller SS
    Invest. Ophthalmol. Vis. Sci., 2007-12-01;48(12):5722-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Binding of ras to phosphoinositide 3-kinase p110alpha is required for ras-driven tumorigenesis in mice.
    Authors: Gupta S, Ramjaun AR, Haiko P, Wang Y, Warne PH, Nicke B, Nye E, Stamp G, Alitalo K, Downward J
    Cell, 2007-06-01;129(5):957-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines.
    Authors: Rhodes KE, Raivich G, Fawcett JW
    Neuroscience, 2006-04-21;140(1):87-100.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  12. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005-04-01;203(1):35-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human PDGF-AB Protein, CF
By Anonymous on 12/15/2017
Application: Binding assay/Protein-protein interaction