Recombinant Human Plexin A3 His-tag Protein, CF Summary
Product Specifications
Asn20-Ala1220, with a C-terminal 6-His tag
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10519-PA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Human Plexin A3 His-tag Protein (10519-PA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 135-160 kDa.
Reconstitution Calculator
Background: Plexin A3
Plexin A3, also known as Plexin-4, is a member of the large Plexin family of conserved transmembrane receptors that mediate Semaphorin functions (1, 2). There are four classes plexins (A, B, C, and D) and they share a similar domain structure: An extracellular domain (ECD) that binds the semaphorin ligand, a single transmembrane domain and an approximately 600 residue-long cytoplasmic region (2). The ECD of mature, human plexinA-3 consists of a Sema domain and four IPT (Ig-like, plexins, transcription factors) domains and shares 93% amino acid identity with both mouse and rat PlexinA-3. Plexin A3 is a coreceptor for class 3 semaphorins and plays an important role in axon guidance in the developing nervous system and regulates the migration of sympathetic neurons (3, 4). Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin (4). Plexin A3 is required for normal dendrite spine morphology in pyramidal neurons and may play a role in regulating semaphorin-mediated programmed cell death in the developing nervous system (5). The cytoplasmic domain of plexinA-3 is required for the activation of down-stream signaling events in the cytoplasm (6).
- Pascoe, H.G. et al. (2015) Prog Biophys Mol Biol. 118(3):161.
- Tamagnone L et al. (1999) Cell. 99:71.
- Alto, L. T. et al. (2017) Methods Mol Biol. 1493:1.
- Yaron, A. et al. (2005) Neuron. 45:513.
- Cheng, H. J. et al. (2001) Neuron. 32:249.
- He H. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:15610.
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