Recombinant Human Plexin A4 Protein, CF
Recombinant Human Plexin A4 Protein, CF Summary
Product Specifications
Thr24-Pro1237, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5856-PA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human Plexin-A4/C-His (Catalog # 5856-PA) has a molecular weight (MW) of 154.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Reconstitution Calculator
Background: Plexin A4
Plexin A4 is a 220‑230 kDa member of the plexin A subfamily, plexin family of proteins (1). It is found on sensory, autonomic and motor neurons and oligodendrocytes, plus T cells and dendritic cells (1‑8). Mature human Plexin A4 is an 1871 amino acid (aa) type I transmembrane glycoprotein with a 23 aa signal sequence, a 1214 aa extracellular domain (ECD), and a 636 aa cytoplasmic region. The ECD contains one Sema-domain (aa 51‑482), three PSI domains (aa 509‑856) and four IPT regions (aa 858‑1230) that contain a phosphoserine at aa 946 (1). Of three isoform variants, one shows a 65 aa substitution for aa 458‑1894, a second shows an 80 aa substitution for aa 1292‑1894, and a third shows the just mentioned 80 aa substitution coupled to a 14 aa substitution for aa 1‑535 (9). The human Plexin A4 ECD shares 97% aa identity with mouse, equine, canine, and bovine Plexin A4. Full‑length Plexin A4 also shares 67% aa identity with the most related family member, Plexin A2. Plexin A4 regulates cell migration, activation and axon guidance via repulsion (1‑5). It serves as a receptor for transmembrane semaphorins, Sema6A and 6B, and as a coreceptor with neuropilin-1 for the secreted semaphorin, Sema3A (1‑8). During development, it plays a role in nerve migration and midline crossing and down‑regulates dendrite formation (2‑8). It is often co‑expressed with Plexin A3, which can also engage class 6 semaphorins but prefers Sema3F/ neuropilin‑2 to Sema3A/neuropilin-1 (3, 8). Thus, Plexins A3 and A4 are redundant in some functions, but unique in others. In T cells, Plexin A4 engages Sema3A and negatively regulates TCR signals (6).
- Suto, F. et al. (2003) Mech. Dev. 120:385.
- Suto, F. et al. (2005) J. Neurosci. 25:3628.
- Faulkner, R.L. et al. (2008) Neural Dev. 3:21.
- Waimey, K.E. et al. (2008) Dev. Biol. 315:448.
- Runker, A.E. et al. (2008) Neural Dev. 3:34.
- Yamamoto, M. et al. (2008) Int. Immunol. 20:413.
- Okada, A. et al. (2007) Biochem. Biophys. Res. Commun. 352:158.
- Yaron, A. et al. (2005) Neuron 45:513.
- Protein Accession # NP_861440, EAW83796, EAL24077.
Citation for Recombinant Human Plexin A4 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Plexin-A4 mediates amyloid-&beta-induced tau pathology in Alzheimer's disease animal model
Authors: S Chung, J Yang, HJ Kim, EM Hwang, W Lee, K Suh, H Choi, I Mook-Jung
Progress in neurobiology, 2021-05-15;0(0):102075.
Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay
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